Linked Life Data

8870848

Source:http://linkedlifedata.com/resource/pubmed/id/8870848

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
1997-1-13
pubmed:abstractText
We used semiquantitative RT-PCR to monitor the expression of mRNA encoding cytokines (IL-1 beta, IL-6, TNF-alpha, and IL-10) and IFN-gamma in fresh isolated peripheral blood mononuclear cells (PBMCs), lymph node mononuclear cells (LNMCs), and mononuclear cells obtained after bronchoalveolar lavages (BALMCs), of four cynomolgus macaques inoculated intravenously with a pathogenic isolate of simian immunodeficiency virus (SIVmac251). To investigate the effects of the viral load on the expression of the cytokines, two monkeys received 30 mg kg-1 day-1 of didanosine (ddI). The two nontreated monkeys became infected and seroconverted, whereas the ddI-treated monkeys were completely protected as demonstrated by all criteria of diagnosis of SIV infection. Concomitant with the peak of viral replication (2 weeks after the experimental inoculation), high levels of IL-6 mRNA were produced in PBMCs, LNMCs, and BALMCs of the two placebotreated infected monkeys. Overexpression of TNF-alpha and IL-10 mRNAs was sometimes observed in LNMCs and BALMCs. A progressive overexpression of IFN-gamma mRNA, starting 2 weeks after experimental inoculation, was observed in BALMCs from infected animals. Concurrently, a marked increase in the CD8+ lymphocyte percentage in the BAL fluids was detected by FACS analysis. Thus, our results emphasize the importance of a comparative study of the expression of cytokines in different tissues. They suggest the interactions of monocyte/macrophage monokine production with viral replication, as well as the role of IFN-gamma in the development of lung cellular immunity to SIV infection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0889-2229
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1263-72
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8870848-Acute Disease, pubmed-meshheading:8870848-Animals, pubmed-meshheading:8870848-Antibodies, Viral, pubmed-meshheading:8870848-Antiviral Agents, pubmed-meshheading:8870848-CD4-Positive T-Lymphocytes, pubmed-meshheading:8870848-CD8-Positive T-Lymphocytes, pubmed-meshheading:8870848-Cell Line, pubmed-meshheading:8870848-Cytokines, pubmed-meshheading:8870848-Didanosine, pubmed-meshheading:8870848-Female, pubmed-meshheading:8870848-Follow-Up Studies, pubmed-meshheading:8870848-Gene Expression, pubmed-meshheading:8870848-HIV Core Protein p24, pubmed-meshheading:8870848-Interferon-gamma, pubmed-meshheading:8870848-Interleukin-1, pubmed-meshheading:8870848-Interleukin-10, pubmed-meshheading:8870848-Interleukin-6, pubmed-meshheading:8870848-Leukocytes, Mononuclear, pubmed-meshheading:8870848-Macaca fascicularis, pubmed-meshheading:8870848-Male, pubmed-meshheading:8870848-RNA, Messenger, pubmed-meshheading:8870848-Simian Acquired Immunodeficiency Syndrome, pubmed-meshheading:8870848-Simian immunodeficiency virus, pubmed-meshheading:8870848-Tumor Necrosis Factor-alpha
pubmed:year
1996
pubmed:articleTitle
Cytokine mRNA expression in mononuclear cells from different tissues during acute SIVmac251 infection of macaques.
pubmed:affiliation
Service de Neurovirologie, Centre de Recherches du Service de Santé des Armées Emile Pardé, Commissariat à l'Energie Atomique, DSV/DRM, Institut Paris-Sud sur les Cytokines, Fontenay aux Roses, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't