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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1996-12-4
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pubmed:abstractText |
Stimulation of several human and murine hematopoietically derived cell lines with anti-Fas antibodies induced increased tyrosine phosphorylation of a panel of proteins observed in whole-cell lysates. In the human T cell line Jurkat, the activity of a 56-kDa tyrosine kinase was likewise activated by anti-Fas antibodies. Immunoprecipitation studies of anti-Fas-stimulated human Jurkat and murine 2B4.11 T cells revealed activation of the Src-family tyrosine kinases Lck and Fyn. Fas receptor-induced tyrosine phosphorylation of p120 c-cbl proto-oncogene product was observed in Jurkat T cells. Pharmacological experiments demonstrated that pretreatment of Jurkat cells with tyrphostins inhibited Fas-induced apoptosis; likewise, Lck activity was inhibited by tyrphostins in a dose-dependent fashion. Finally, Lck derived from unstimulated Jurkat T cells formed stable complexes with the intracellular domain of the Fas receptor. These data are consistent with the notion that expression and activation of members of the Src-family kinases is required for Fas-induced cell death in T lymphocytes and consistent with recent findings demonstrating decreased Fas-mediated thymocytic death in Fyn-knockout mice.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/CBL protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cbl protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/FYN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fyn protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Genistein,
http://linkedlifedata.com/resource/pubmed/chemical/Isoflavones,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Specific Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-cbl,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fyn,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Protein Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/src-Family Kinases
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0741-5400
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
546-54
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8864141-Animals,
pubmed-meshheading:8864141-Antigens, CD95,
pubmed-meshheading:8864141-Apoptosis,
pubmed-meshheading:8864141-Cells, Cultured,
pubmed-meshheading:8864141-Enzyme Inhibitors,
pubmed-meshheading:8864141-Genistein,
pubmed-meshheading:8864141-Humans,
pubmed-meshheading:8864141-Isoflavones,
pubmed-meshheading:8864141-Lymphocyte Specific Protein Tyrosine Kinase p56(lck),
pubmed-meshheading:8864141-Mice,
pubmed-meshheading:8864141-Monocytes,
pubmed-meshheading:8864141-Phosphorylation,
pubmed-meshheading:8864141-Phosphotyrosine,
pubmed-meshheading:8864141-Protein-Tyrosine Kinases,
pubmed-meshheading:8864141-Proto-Oncogene Proteins,
pubmed-meshheading:8864141-Proto-Oncogene Proteins c-cbl,
pubmed-meshheading:8864141-Proto-Oncogene Proteins c-fyn,
pubmed-meshheading:8864141-Signal Transduction,
pubmed-meshheading:8864141-T-Lymphocytes,
pubmed-meshheading:8864141-Ubiquitin-Protein Ligases,
pubmed-meshheading:8864141-src-Family Kinases
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pubmed:year |
1996
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pubmed:articleTitle |
Activation of Src-family tyrosine kinases during Fas-induced apoptosis.
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pubmed:affiliation |
Department of Microbiology, Ohio State University, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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