Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1997-2-19
pubmed:abstractText
Proinsulin is converted to mature insulin by two reactions, cleavage by the prohormone convertases PC2 and PC3, and removal of basic residues by carboxypeptidase H. These reactions are performed in the secretory granules of pancreatic beta cells. When we replaced the processing sites of proinsulin with furin-cleavable sites, the three nonneuroendocrine cell lines Hep G2, CHO, and NIH/3T3 produced insulin with the same size as synthetic human insulin. Although the three cell lines expressed different quantities of carboxypeptidase H mRNA, the cytosol fractions of the cells exhibited similar levels of carboxypeptidase activity, suggesting that additional carboxypeptidases were active. The insulins resulting from the three cell lines were eluted as a single peak on a cation-exchange chromatography column, indicating that proinsulin can be maturated to insulin even in nonneuroendocrine cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0196-9781
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
789-96
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:8844768-3T3 Cells, pubmed-meshheading:8844768-Animals, pubmed-meshheading:8844768-Blotting, Northern, pubmed-meshheading:8844768-CHO Cells, pubmed-meshheading:8844768-Carboxypeptidase H, pubmed-meshheading:8844768-Carboxypeptidases, pubmed-meshheading:8844768-Cattle, pubmed-meshheading:8844768-Cell Line, pubmed-meshheading:8844768-Chromatography, Gel, pubmed-meshheading:8844768-Chromatography, Ion Exchange, pubmed-meshheading:8844768-Cricetinae, pubmed-meshheading:8844768-Culture Media, Serum-Free, pubmed-meshheading:8844768-Cytosol, pubmed-meshheading:8844768-Furin, pubmed-meshheading:8844768-Gene Expression, pubmed-meshheading:8844768-Humans, pubmed-meshheading:8844768-Insulin, pubmed-meshheading:8844768-Islets of Langerhans, pubmed-meshheading:8844768-Mice, pubmed-meshheading:8844768-Proinsulin, pubmed-meshheading:8844768-RNA, Messenger, pubmed-meshheading:8844768-Rats, pubmed-meshheading:8844768-Recombinant Proteins, pubmed-meshheading:8844768-Subtilisins, pubmed-meshheading:8844768-Transfection
pubmed:year
1996
pubmed:articleTitle
Proinsulin cleaved by furin is processed to chromatographically mature insulin by carboxypeptidases in nonneuroendocrine cells.
pubmed:affiliation
Department of Molecular Medicine, Gunma University, Maebashi, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't