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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-9-23
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pubmed:abstractText |
We have shown previously that dendritic cells (DC) produce IL-12 upon interaction with CD4+ T cells. Here we ask how this IL-12 production is induced and regulated. Quantitative PCR and in situ hybridization for IL-12 p40 and an ELISA specific for the p70 heterodimer were used to determine IL-12 production. We demonstrate that ligation of either CD40 or MHC class II molecules independently trigger IL-12 production in DC, and that IL-12 production is downregulated by IL-4 and IL-10. The levels of bioactive IL-12 that can be released by triggering with an anti-CD40 mAb or with a T cell hybridoma are high (range 260-4700 pg/ml from 1 X 10(6) DC in 72 h). The CD40-mediated pathway indicates that IL-12 production is induced in DC upon interaction with activated, CD40 ligand-expressing helper T cells, even in the absence of cognate antigen recognition. Side-by-side comparison of IL-12 production, and blocking experiments employing an anti-CD40 ligand mAb, suggest that the CD40-mediated pathway is quantitatively more significant than induction via the MHC class II molecule. The importance of the CD40/CD40 ligand interaction for IL-12 induction in DC likely contributes to the recent finding that mice lacking the CD40 ligand are impaired in mounting Th1 type cell-mediated immune responses.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-1350290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-1375245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-1656278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-1694219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-2910499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-3871837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-7516669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-7523569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-7537673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-7594465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-7612223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-7614984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-7730613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-7836910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-7904900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-8102388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-8145053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-8568240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-8576576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-8605935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8760828-8621933
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
184
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
741-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8760828-Animals,
pubmed-meshheading:8760828-Antigens, CD40,
pubmed-meshheading:8760828-Base Sequence,
pubmed-meshheading:8760828-CD4-Positive T-Lymphocytes,
pubmed-meshheading:8760828-DNA Primers,
pubmed-meshheading:8760828-Dendritic Cells,
pubmed-meshheading:8760828-Down-Regulation,
pubmed-meshheading:8760828-Female,
pubmed-meshheading:8760828-Histocompatibility Antigens Class II,
pubmed-meshheading:8760828-Immunity, Cellular,
pubmed-meshheading:8760828-Interleukin-10,
pubmed-meshheading:8760828-Interleukin-12,
pubmed-meshheading:8760828-Interleukin-4,
pubmed-meshheading:8760828-Male,
pubmed-meshheading:8760828-Mice,
pubmed-meshheading:8760828-Mice, Inbred BALB C,
pubmed-meshheading:8760828-Mice, Inbred C3H,
pubmed-meshheading:8760828-Mice, Inbred C57BL,
pubmed-meshheading:8760828-Molecular Sequence Data,
pubmed-meshheading:8760828-Spleen,
pubmed-meshheading:8760828-Up-Regulation
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pubmed:year |
1996
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pubmed:articleTitle |
High level IL-12 production by murine dendritic cells: upregulation via MHC class II and CD40 molecules and downregulation by IL-4 and IL-10.
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pubmed:affiliation |
Department of Dermatology, University of Innsbruck, Austria.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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