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rdf:type |
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lifeskim:mentions |
umls-concept:C0000781,
umls-concept:C0006304,
umls-concept:C0017262,
umls-concept:C0026473,
umls-concept:C0035647,
umls-concept:C0036536,
umls-concept:C0036537,
umls-concept:C0042210,
umls-concept:C0063695,
umls-concept:C0086418,
umls-concept:C0123759,
umls-concept:C0185117,
umls-concept:C0205148,
umls-concept:C0205263,
umls-concept:C1948023,
umls-concept:C2349975,
umls-concept:C2911684
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pubmed:issue |
8
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pubmed:dateCreated |
1996-9-26
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pubmed:abstractText |
Development of a vaccine which is capable of generating a strong cellular immune response associated with gamma interferon (IFN-gamma) production and cytotoxic T-cell development requires that the immunogen be capable of inducing the secretion of interleukin-12 (IL-12), which is a pivotal factor for the differentiation of Th1 or Tc1 cells. We have previously shown that the heat-inactivated gram-negative bacterium Brucella abortus can induce IFN-gamma secretion by T cells. In the present study, we demonstrate that B. abortus and lipopolysaccharide (LPS) from B. abortus can induce IL-12 p40 mRNA expression and protein secretion by human elutriated monocytes (99% pure). p40 mRNA was detected within 4 h, and p40 protein could be measured at 24 h. This induction was abrogated by anti-CD14 monoclonal antibody, suggesting that monocytes recognize B. abortus via their receptor for LPS. The biological activity of IL-12 secreted by B. abortus-stimulated monocytes was demonstrated by its ability to upregulate IFN-gamma mRNA expression in T cells separated from monocytes and B. abortus by a transwell membrane. The B. abortus-induced IL-12 also enhanced NK cytolytic activity against K562 target cells. B. abortus was shown to rapidly increase the expression of the costimulatory molecules B7.1 and B7.2 and intercellular adhesion molecule 1 on human monocytes. Together, these data indicate that B. abortus can directly activate human monocytes and provide the cytokine milieu which would direct the immune response towards Th1-Tc1 differentiation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1357073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1358972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1360994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1381594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1383317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1548064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1578138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1578139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1650475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1673147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1698311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1701314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1713608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1847722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-1972160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-2204066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-2504877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-3107127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-3125247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-3127461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-3918102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7512027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7516409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7525836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7527811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7532678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7536798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7538442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7589116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7594526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7605994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7637808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7722441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7745677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7790090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7796298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7809096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7843232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7901851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7904381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7907107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7908322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7908633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7908634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7908914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7909320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7917115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7925555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-7938013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-8096238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-8102388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-8104472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-8392095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-8627787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8757841-99320
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/CD86 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Inactivated
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0019-9567
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pubmed:author |
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