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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-10-16
|
| pubmed:abstractText |
Fresh cytosols extracted from unfertilised eggs of Xenopus laevis contain a cytostatic factor (CSF) which arrests the cell cycle at metaphase when microinjected into cleaving blastomeres. This CSF is sensitive to Ca2+ and is designated primary CSF (1 degrees CSF). During storage of Ca2(+)-containing cytosols at 2 degrees C, a stable CSF activity appears which is designated secondary CSF (2 degrees CSF). In the present study, we report that 2 degrees CSF activity can be induced in cytosols extracted from stage VI oocytes, unfertilised eggs, electrically activated eggs or blastulae, in the presence of Ca2+. Both the intensity and the rate of 2 degrees CSF development are dependent on the concentration of Ca2+ ions added to the cytosol. At Ca2+ concentrations of 5-10 mM, 2 degrees CSF activity reaches a maximum in about 7 days. Secondary CSF is relatively resistant to heat but loses all activity after 5 min at 70 degrees C. When stored at-80 degrees C, 2 degrees CSF activity remains detectable for about 6 weeks. Cytological observations show that blastomeres arrested by microinjection of 2 degrees CSF developed in cytosols of unfertilised eggs, activated eggs or blastulae contain metaphase chromosomes embedded in a bipolar spindle that has no asters developed at its poles. In contrast, blastomeres arrested by 2 degrees CSF in cytosols of stage VI oocytes contain condensed chromosomes but no spindle is formed. The mechanisms of the development of 2 degrees CSF in Ca2(+)-containing cytosols and its mode of action are discussed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0967-1994
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
11-9
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading | |
| pubmed:year |
1996
|
| pubmed:articleTitle |
Calcium-dependent development of secondary cytostatic factor (2 degrees CSF) from Xenopus laevis oocytes and zygotes.
|
| pubmed:affiliation |
Department of Zoology, University of Toronto, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|