Linked Life Data

8727522

Source:http://linkedlifedata.com/resource/pubmed/id/8727522

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-2-6
pubmed:abstractText
Metal-binding proteins were isolated from ovaries of dab (Limanda limanda L.) caught in the North Sea. Separation of the heat-treated cytosolic fraction of dab ovaries by gel-filtration chromatography revealed the presence of a major zinc-binding protein with a low-molecular-weight of 10,000-12,000 Da, similar to mammalian metallothionein (MT). The metal-binding protein was analyzed by anion-exchange chromatography and by reversedphase high-performance liquid chromatography. The metal-binding protein from dab ovaries consists of one major isoform. Zinc could be displaced from the isoform by a saturation of the extract with cadmium, leading to altered retention of the protein at the RP column. In a laboratory experiment, dabs were treated intraperitoneally with cadmium (0.5 and 1.5 mg/kg body wt). In contrast to hepatic dab MT, the metal-binding protein in ovaries was not inducable by cadmium; however, dose-dependent amounts of cadmium were bound to the ovarian protein after the metal exposure. The amino acid composition was different from that of mammalian MT. The results suggest that the metal-binding protein in dab ovaries is possibly involved in ovary development and can be affected by cadmium exposure.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0147-6513
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Isolation of a metal-binding protein from ovaries of dab (Limanda limanda L.) distinct from metallothionein: effect of cadmium exposure.
pubmed:affiliation
Institut für Biochemie und Lebensmittelchemie, Universität Hamburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't