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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0019691,
umls-concept:C0019704,
umls-concept:C0024432,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0205225,
umls-concept:C0205227,
umls-concept:C0205263,
umls-concept:C0333516,
umls-concept:C1332714,
umls-concept:C1422036,
umls-concept:C2911684
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pubmed:issue |
1
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pubmed:dateCreated |
1996-9-12
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pubmed:abstractText |
Among immunological abnormalities present in human immunodeficiency virus type 1 (HIV-1)-infected individuals are dysregulation of cytokine production and CD4 down-regulation in both T-helper cells and monocytes/macrophages. The HIV-1 envelope glycoprotein 120 (gp120) has the ability to induce different cytokines in peripheral blood mononuclear cells and in monocytes/macrophages in vitro which in some instances have been reported to down-regulate macrophage CD4 expression. This study provides evidence that HIV-1 recombinant gp120 (rgp120) down-regulates both surface and total CD4 expression in primary tissue culture-differentiated macrophages (TCDM) at the level of transcription. The CD4 down-regulation observed in TCDM occurred between 6 and 12 hr after rgp120 treatment preceded by a peak of endogenous tumour necrosis factor-alpha (TNF-alpha) observed at 3-6 hr post-treatment. We demonstrate that the TCDM CD4 down-regulation observed after rgp120 treatment was inhibited by the use of an anti-huTNF-alpha monoclonal antibody (mAb), but not by mAb directed against other cytokines induced by rgp120, such as interleukin-1 beta (IL-1 beta) and interferon-alpha (IFN-alpha). The present findings roughly parallel those observed both in the sera of patients and in the monocytes/macrophages isolated from HIV-positive individuals, suggesting that gp120 by stimulating endogenous TNF-alpha production could be a good candidate for the CD4 down-regulation observed in the monocytes/macrophages of HIV-1-infected individuals. In contrast to CD4 down-regulation in HIV-infected lymphocytes, which results from a direct effect of viral genes on CD4 expression, soluble factors such as cytokines induced during HIV infection might explain the monocyte/macrophage CD4 dysregulation observed in acquired immune deficiency syndrome.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-1694679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-1740750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-1918997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-1920639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-2114424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-2397055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-2492910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-2536171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-2541200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-2677236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-2789293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-2868277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-3107214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-3414726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-6410234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-7511077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-7511078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-7526537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-7527834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-7586702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-7904170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-7991540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-8096699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-8145026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-8230446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-8235617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-8409454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-8446772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-8516299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8707350-94549
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
55-60
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8707350-Antibodies, Monoclonal,
pubmed-meshheading:8707350-Antigens, CD4,
pubmed-meshheading:8707350-Base Sequence,
pubmed-meshheading:8707350-Cells, Cultured,
pubmed-meshheading:8707350-DNA Primers,
pubmed-meshheading:8707350-Down-Regulation,
pubmed-meshheading:8707350-Flow Cytometry,
pubmed-meshheading:8707350-HIV Envelope Protein gp120,
pubmed-meshheading:8707350-Humans,
pubmed-meshheading:8707350-Macrophages,
pubmed-meshheading:8707350-Molecular Sequence Data,
pubmed-meshheading:8707350-Polymerase Chain Reaction,
pubmed-meshheading:8707350-Recombinant Proteins,
pubmed-meshheading:8707350-Stimulation, Chemical,
pubmed-meshheading:8707350-Time Factors,
pubmed-meshheading:8707350-Tumor Necrosis Factor-alpha
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pubmed:year |
1996
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pubmed:articleTitle |
HIV-1 envelope glycoprotein gp120 down-regulates CD4 expression in primary human macrophages through induction of endogenous tumour necrosis factor-alpha.
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pubmed:affiliation |
I.N.S.E.R.M. Unit 74, Louis Pasteur University, Strasbourg, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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