| pubmed-article:8703996 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8703996 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:8703996 | lifeskim:mentions | umls-concept:C0063695 | lld:lifeskim |
| pubmed-article:8703996 | lifeskim:mentions | umls-concept:C0070750 | lld:lifeskim |
| pubmed-article:8703996 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8703996 | pubmed:dateCreated | 1996-9-10 | lld:pubmed |
| pubmed-article:8703996 | pubmed:abstractText | The effect of phorbol 12-myristate 13-acetate (PMA) on the expression and shedding of intercellular adhesion molecule-1 (ICAM-1) was investigated on the hematopoietic cell lines K 562 and U 937 using flow cytometry, fluorescence microscopy and ELISA technique. At low concentration of 1 nM, PMA stimulated the expression of ICAM-1 on the cell surface about 4-fold within 24 h, whereas a short-term treatment with 100 nM PMA led to the shedding of 35% of ICAM-1 from the surface of K 562 cells. The release of surface ICAM-1 was found on single cells by fluorescence microscopy to be a uniform process proceeding within 15 min. The shedding of ICAM-1 correlated with elevated levels of sICAM-1 in the supernatants of cultured cells. Also on K 562 cells stimulated by TNF-alpha, a PMA-induced release of ICAM-1 was observed in addition to the known spontaneous shedding. In contrast to the results with K 562 cells, no PMA-induced shedding of ICAM-1 was found on U 937 cells. This indicates a cell-specific process for K 562 cells. The PMA-mediated release of ICAM-1 from K 562 cells suggests that the shedding process does not only occur in parallel to the surface expression of ICAM-1, but may be controlled by particular mechanisms of down-regulation. | lld:pubmed |
| pubmed-article:8703996 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8703996 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8703996 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8703996 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8703996 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8703996 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8703996 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8703996 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:8703996 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:8703996 | pubmed:author | pubmed-author:BohnensackRR | lld:pubmed |
| pubmed-article:8703996 | pubmed:author | pubmed-author:AnsorgeSS | lld:pubmed |
| pubmed-article:8703996 | pubmed:author | pubmed-author:SchönfeldPP | lld:pubmed |
| pubmed-article:8703996 | pubmed:author | pubmed-author:FritzSS | lld:pubmed |
| pubmed-article:8703996 | pubmed:author | pubmed-author:ReinholdDD | lld:pubmed |
| pubmed-article:8703996 | pubmed:author | pubmed-author:SchlüterTT | lld:pubmed |
| pubmed-article:8703996 | pubmed:author | pubmed-author:StriggowFF | lld:pubmed |
| pubmed-article:8703996 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8703996 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:8703996 | pubmed:volume | 1312 | lld:pubmed |
| pubmed-article:8703996 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8703996 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8703996 | pubmed:pagination | 255-61 | lld:pubmed |
| pubmed-article:8703996 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:8703996 | pubmed:meshHeading | pubmed-meshheading:8703996-... | lld:pubmed |
| pubmed-article:8703996 | pubmed:meshHeading | pubmed-meshheading:8703996-... | lld:pubmed |
| pubmed-article:8703996 | pubmed:meshHeading | pubmed-meshheading:8703996-... | lld:pubmed |
| pubmed-article:8703996 | pubmed:meshHeading | pubmed-meshheading:8703996-... | lld:pubmed |
| pubmed-article:8703996 | pubmed:meshHeading | pubmed-meshheading:8703996-... | lld:pubmed |
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| pubmed-article:8703996 | pubmed:meshHeading | pubmed-meshheading:8703996-... | lld:pubmed |
| pubmed-article:8703996 | pubmed:meshHeading | pubmed-meshheading:8703996-... | lld:pubmed |
| pubmed-article:8703996 | pubmed:meshHeading | pubmed-meshheading:8703996-... | lld:pubmed |
| pubmed-article:8703996 | pubmed:meshHeading | pubmed-meshheading:8703996-... | lld:pubmed |
| pubmed-article:8703996 | pubmed:meshHeading | pubmed-meshheading:8703996-... | lld:pubmed |
| pubmed-article:8703996 | pubmed:meshHeading | pubmed-meshheading:8703996-... | lld:pubmed |
| pubmed-article:8703996 | pubmed:meshHeading | pubmed-meshheading:8703996-... | lld:pubmed |
| pubmed-article:8703996 | pubmed:meshHeading | pubmed-meshheading:8703996-... | lld:pubmed |
| pubmed-article:8703996 | pubmed:meshHeading | pubmed-meshheading:8703996-... | lld:pubmed |
| pubmed-article:8703996 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8703996 | pubmed:articleTitle | Phorbol ester-induced shedding of intercellular adhesion molecule-1 (ICAM-1) on erythroleukemic K 562 cells. | lld:pubmed |
| pubmed-article:8703996 | pubmed:affiliation | Institut für Biochemie, Otto-von-Guericke Universität Magdeburg, Germany. | lld:pubmed |
| pubmed-article:8703996 | pubmed:publicationType | Journal Article | lld:pubmed |
