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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1996-9-10
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pubmed:abstractText |
The effect of phorbol 12-myristate 13-acetate (PMA) on the expression and shedding of intercellular adhesion molecule-1 (ICAM-1) was investigated on the hematopoietic cell lines K 562 and U 937 using flow cytometry, fluorescence microscopy and ELISA technique. At low concentration of 1 nM, PMA stimulated the expression of ICAM-1 on the cell surface about 4-fold within 24 h, whereas a short-term treatment with 100 nM PMA led to the shedding of 35% of ICAM-1 from the surface of K 562 cells. The release of surface ICAM-1 was found on single cells by fluorescence microscopy to be a uniform process proceeding within 15 min. The shedding of ICAM-1 correlated with elevated levels of sICAM-1 in the supernatants of cultured cells. Also on K 562 cells stimulated by TNF-alpha, a PMA-induced release of ICAM-1 was observed in addition to the known spontaneous shedding. In contrast to the results with K 562 cells, no PMA-induced shedding of ICAM-1 was found on U 937 cells. This indicates a cell-specific process for K 562 cells. The PMA-mediated release of ICAM-1 from K 562 cells suggests that the shedding process does not only occur in parallel to the surface expression of ICAM-1, but may be controlled by particular mechanisms of down-regulation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
1312
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
255-61
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:8703996-Blotting, Western,
pubmed-meshheading:8703996-Cell Line,
pubmed-meshheading:8703996-Dose-Response Relationship, Drug,
pubmed-meshheading:8703996-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8703996-Flow Cytometry,
pubmed-meshheading:8703996-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:8703996-Gene Expression,
pubmed-meshheading:8703996-Humans,
pubmed-meshheading:8703996-Intercellular Adhesion Molecule-1,
pubmed-meshheading:8703996-Kinetics,
pubmed-meshheading:8703996-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:8703996-Microscopy, Fluorescence,
pubmed-meshheading:8703996-Tetradecanoylphorbol Acetate,
pubmed-meshheading:8703996-Tumor Cells, Cultured,
pubmed-meshheading:8703996-Tumor Necrosis Factor-alpha
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pubmed:year |
1996
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pubmed:articleTitle |
Phorbol ester-induced shedding of intercellular adhesion molecule-1 (ICAM-1) on erythroleukemic K 562 cells.
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pubmed:affiliation |
Institut für Biochemie, Otto-von-Guericke Universität Magdeburg, Germany.
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pubmed:publicationType |
Journal Article
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