Linked Life Data

8703974

Source:http://linkedlifedata.com/resource/pubmed/id/8703974

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-9-6
pubmed:abstractText
The transport of L-glutamate by lactating rat mammary gland has been examined using both tissue explants and a perfused mammary preparation. L-Glutamate uptake by mammary tissue explants was predominantly via a Na(+)-dependent pathway: Li+, choline+ and NMDG+ could not substitute for Na+. L-Glutamate efflux from preloaded explants was also influenced by the transmembrane Na(+)-gradient. These results are consistent with (Na(+)-glutamate) cotransport. The Na(+)-dependent system for L-glutamate transport in tissue explants was saturable (Km = 112.5 +/- 19.7 microM; Vmax = 71.3 +/- 10.4 nmol/min per g cells) and selective for anionic amino acids. Thus, D- and L-aspartate were high affinity inhibitors of L-glutamate uptake whereas neutral amino acids were relatively ineffective. D-Aspartate inhibited L-glutamate uptake in a competitive fashion. L-Glutamate uptake by the perfused mammary gland was (a) Na(+)-dependent (b) saturable (Km = 18.1 +/- 4.9 microM; Vmax = 40.3 +/- 3.7 nmol/min per g tissue) and (c) selective for anionic amino acids. The results suggest that the (Na(+)-glutamate) cotransporter is situated in the blood-facing aspect of the mammary epithelium.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
1282
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
200-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
The mechanism of L-glutamate transport by lactating rat mammary tissue.
pubmed:affiliation
Hannah Research Institute, Ayr, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't