Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-8-8
|
| pubmed:abstractText |
Elevated levels of soluble intercellular adhesion molecule-1 have been shown predictive of post-injury multiple organ failure. We hypothesized that sICAM-1 augments distant organ injury via its affect on the PMN and; thus, have examined neutrophil elastase and superoxide production in response to sICAM-1. To obtain soluble ICAM-1, Chinese Hamster Ovarian (CHO) cells were transfected with human ICAM-1 (cDNA vector CD1.8), lysed and centrifuged at 150,000g for 1 hr; supernatant was passed over an ICAM-1 affinity gradient, eluted with 0.1 mM glycine x HCl, and concentrated using an Amicon Spin-X filter. PMNs were incubated for 1 hr with sICAM-1 at 37 degrees C. Quiescent and PMA-activated PMNs served as negative and positive controls respectively. Elastase activity was measured by the cleavage of methoxy-succinyl-alalyl-alalyl-prolyl-valyl-p-nitroanilide. Superoxide production was determined by superoxide dismutase inhibitive ferricytochrome C reduction over a 5-60 min incubation. PMN incubation with sICAM-1 provoked marked increase in elastase release 10.43 +/- 2.90 (10(-6) U/hr) compared to control 1.64 +/- 0.57, and was equivalent to PMA-activated PMN elastase release 11.60 +/- 1.50 (10(-6) U/hr). In contrast, sICAM-1 alone did not promote spontaneous PMN superoxide production beyond buffer treated PMNs (0.25 +/- 0.09 nmole/2.5 x 10(5) PMN/min). In sum, sICAM-1 stimulates PMN elastase release in vitro. Clinically, this may represent a mechanism by which sICAM-1 participates in the genesis of post-injury multiple organ failure.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Leukocyte Elastase,
http://linkedlifedata.com/resource/pubmed/chemical/Pancreatic Elastase,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxides,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0022-4804
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
63
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
6-10
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8661163-Amino Acid Sequence,
pubmed-meshheading:8661163-Animals,
pubmed-meshheading:8661163-CHO Cells,
pubmed-meshheading:8661163-Chromatography, Affinity,
pubmed-meshheading:8661163-Cricetinae,
pubmed-meshheading:8661163-Endothelium, Vascular,
pubmed-meshheading:8661163-Humans,
pubmed-meshheading:8661163-Intercellular Adhesion Molecule-1,
pubmed-meshheading:8661163-Leukocyte Elastase,
pubmed-meshheading:8661163-Models, Biological,
pubmed-meshheading:8661163-Molecular Sequence Data,
pubmed-meshheading:8661163-Neutrophils,
pubmed-meshheading:8661163-Pancreatic Elastase,
pubmed-meshheading:8661163-Recombinant Proteins,
pubmed-meshheading:8661163-Substrate Specificity,
pubmed-meshheading:8661163-Superoxides,
pubmed-meshheading:8661163-Tetradecanoylphorbol Acetate,
pubmed-meshheading:8661163-Ultrafiltration
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Soluble ICAM-1 (sICAM-1) provokes PMN elastase release.
|
| pubmed:affiliation |
Department of Surgery, Denver General Hospital and the University of Colorado Health Sciences Center, USA.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
|