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pubmed-article:8647259pubmed:abstractTextGelatinase A is secreted as a proenzyme (progelatinase A) which is activated and bound on the surface of tumor and normal cells. We have reported that the expression of a membrane-type-1-matrix metalloproteinase (MT1-MMP) induces activation of progelatinase A. Here we demonstrate that the expression of MT1-MMP in COS-1 cells induces cell-surface binding of progelatinase A which is consequently processed to an intermediate form. Processing from the intermediate to the fully active form is dependent on the gelatinase A concentration. These results suggest that the cell-surface binding concentrates the gelatinase A intermediate form locally to allow autoproteolytic processing to the fully active form.lld:pubmed
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pubmed-article:8647259pubmed:articleTitleCell surface binding and activation of gelatinase A induced by expression of membrane-type-1-matrix metalloproteinase (MT1-MMP).lld:pubmed
pubmed-article:8647259pubmed:affiliationDepartment of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, Japan. vhsato@icews1.ipc.kanazawa-u.ac.jplld:pubmed
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