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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1996-7-23
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pubmed:abstractText |
Gelatinase A is secreted as a proenzyme (progelatinase A) which is activated and bound on the surface of tumor and normal cells. We have reported that the expression of a membrane-type-1-matrix metalloproteinase (MT1-MMP) induces activation of progelatinase A. Here we demonstrate that the expression of MT1-MMP in COS-1 cells induces cell-surface binding of progelatinase A which is consequently processed to an intermediate form. Processing from the intermediate to the fully active form is dependent on the gelatinase A concentration. These results suggest that the cell-surface binding concentrates the gelatinase A intermediate form locally to allow autoproteolytic processing to the fully active form.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
385
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
238-40
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8647259-Animals,
pubmed-meshheading:8647259-Blotting, Western,
pubmed-meshheading:8647259-Cell Membrane,
pubmed-meshheading:8647259-Collagenases,
pubmed-meshheading:8647259-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8647259-Enzyme Activation,
pubmed-meshheading:8647259-Enzyme Precursors,
pubmed-meshheading:8647259-Gelatinases,
pubmed-meshheading:8647259-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:8647259-Matrix Metalloproteinase 1,
pubmed-meshheading:8647259-Matrix Metalloproteinase 2,
pubmed-meshheading:8647259-Metalloendopeptidases,
pubmed-meshheading:8647259-Protein Binding,
pubmed-meshheading:8647259-Protein Processing, Post-Translational,
pubmed-meshheading:8647259-Transfection,
pubmed-meshheading:8647259-Tumor Cells, Cultured
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pubmed:year |
1996
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pubmed:articleTitle |
Cell surface binding and activation of gelatinase A induced by expression of membrane-type-1-matrix metalloproteinase (MT1-MMP).
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pubmed:affiliation |
Department of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, Japan. vhsato@icews1.ipc.kanazawa-u.ac.jp
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pubmed:publicationType |
Journal Article
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