Linked Life Data

8635526

Source:http://linkedlifedata.com/resource/pubmed/id/8635526

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1996-7-10
pubmed:abstractText
Human umbilical cord blood (UCB) is rich in hematopoietic stem cells and progenitors and recently has been used in the clinic as an alternative source for graft and marrow repopulation. We tried to determine in vitro the roles of wild-type (wt) p53 and wt RB tumor/growth suppressor genes in the regulation of proliferation and maturation of hematopoietic UCB cells. CD34+ cells, isolated from mononuclear cells of UCB, were cultured in semisolid medium under conditions that favor growth of hematopoietic cells. We studied the level of expression of p53 and RB mRNAs and proteins during cell culture by Northern blot and cytofluorometry analysis, respectively. Sense (S), antisense (AS), or scrambled (missense [MS]) p53 and RB oligodeoxynucleotides (ODNs) were used to study the behavior of these cells in the absence of expression of p53 and/or RB. Adequate doses of p53 or RB ODNs inducing maximal inhibitory effect were used to study the behavior of these cells in the absence of expression of p53 and/or RB. Adequate doses of p53 or RB ODNs inducing maximal inhibitory effect with minimal cellular toxicity were determined. Exposure of CD34+ cells to p53 or AS, RB AS, or both p53 and RB AS but not other ODNs (sense or missense) resulted in a significantly increased number of colony-forming units-granulocyte/macrophage (CFU-GM) induced by interleukin-3 (IL-3) and/or granulocyte-macrophage colony-stimulating factor (GM-CSF). The number of erythroid colonies (CFU-E) and burst-forming units (BFU-E) derived from CD34+ cells in the presence of erythropoietin (Epo) was not significantly increased, whereas the number of such colonies was markedly increased in the presence of IL-3 + EPO upon p53 AS and/or RB AS treatment with hypothesis that wt p53 and RB are proliferation suppressor genes that interfere with normal maturation of hematopoietic cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0301-472X
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
702-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8635526-Antigens, CD34, pubmed-meshheading:8635526-Base Sequence, pubmed-meshheading:8635526-Cell Separation, pubmed-meshheading:8635526-Cells, Cultured, pubmed-meshheading:8635526-Colony-Forming Units Assay, pubmed-meshheading:8635526-Erythropoiesis, pubmed-meshheading:8635526-Erythropoietin, pubmed-meshheading:8635526-Fetal Blood, pubmed-meshheading:8635526-Gene Expression, pubmed-meshheading:8635526-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:8635526-Granulocytes, pubmed-meshheading:8635526-Hematopoiesis, pubmed-meshheading:8635526-Humans, pubmed-meshheading:8635526-Interleukin-3, pubmed-meshheading:8635526-Macrophages, pubmed-meshheading:8635526-Megakaryocytes, pubmed-meshheading:8635526-Molecular Sequence Data, pubmed-meshheading:8635526-Oligonucleotides, Antisense, pubmed-meshheading:8635526-RNA, Messenger, pubmed-meshheading:8635526-Retinoblastoma Protein, pubmed-meshheading:8635526-Tumor Suppressor Protein p53
pubmed:year
1996
pubmed:articleTitle
Role of p53 and RB on in vitro growth of normal umbilical cord blood cells.
pubmed:affiliation
Laboratoire de Génétique Cellulaire et Moléculaire, C.H.U. de Poitiers, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't