Linked Life Data

8626521

Source:http://linkedlifedata.com/resource/pubmed/id/8626521

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
1996-6-21
pubmed:abstractText
M-CAT sites are required for the activity of many promoters in cardiac and skeletal muscle. M-CAT binding activity is muscle-enriched, but is found in many tissues and is immunologically related to the HeLa transcription enhancer factor-1 (TEF-1). TEF-1-related cDNAs (RTEF-1) have been cloned from chick heart. RTEF-1 mRNA is muscle-enriched, consistent with a role for RTEF-1 in the regulation of muscle-specific gene expression. Here, we have examined the tissue distribution of TEF-1-related proteins and of M-CAT binding activity by Western analysis and mobility shift polyacrylamide gel electrophoresis. TEF-1-related proteins of 57, 54 and 52 kDa were found in most tissues with the highest levels in muscle tissues. All of these TEF-1-related proteins bound M-CAT DNA and the 57- and 54-kDa TEF-1-related polypeptides were phosphorylated. Proteolytic digestion mapping showed that the 54-kDa TEF-1-related polypeptide is encoded by a different gene than the 52- and 57-kDa TEF-1-related polypeptides. A comparison of the migration and proteolytic digestion of the 54-kDa TEF-1-related polypeptide with proteins encoded by the cloned RTEF-1 cDNAs showed that the 54-kDa TEF-1-related polypeptide is encoded by RTEF-1A. High resolution mobility shift polyacrylamide gel electrophoresis showed multiple M-CAT binding activities in tissues. All of these activities contained TEF-1-related proteins. One protein-M-CAT DNA complex was muscle-enriched and was up-regulated upon differentiation of a skeletal muscle cell line. This complex contained the 54-kDa TEF-1-related polypeptide. Therefore, RTEF1-A protein is a component of a muscle-enriched transcription complex that forms on M-CAT sites and may play a key role in the regulation of transcription in muscle.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
271
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8266-74
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8626521-Amino Acid Sequence, pubmed-meshheading:8626521-Animals, pubmed-meshheading:8626521-Chickens, pubmed-meshheading:8626521-DNA-Binding Proteins, pubmed-meshheading:8626521-Gene Expression Regulation, pubmed-meshheading:8626521-Genes, pubmed-meshheading:8626521-Immunologic Techniques, pubmed-meshheading:8626521-Molecular Sequence Data, pubmed-meshheading:8626521-Molecular Weight, pubmed-meshheading:8626521-Multigene Family, pubmed-meshheading:8626521-Muscles, pubmed-meshheading:8626521-Nuclear Proteins, pubmed-meshheading:8626521-Peptide Mapping, pubmed-meshheading:8626521-Phosphoproteins, pubmed-meshheading:8626521-Phosphorylation, pubmed-meshheading:8626521-Promoter Regions, Genetic, pubmed-meshheading:8626521-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:8626521-Tissue Distribution, pubmed-meshheading:8626521-Transcription Factors
pubmed:year
1996
pubmed:articleTitle
The role of transcription enhancer factor-1 (TEF-1) related proteins in the formation of M-CAT binding complexes in muscle and non-muscle tissues.
pubmed:affiliation
Department of Anatomy and Cardiovascular Research Institute, University of California, San Francisco, 94143, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.