Linked Life Data

8625910

Source:http://linkedlifedata.com/resource/pubmed/id/8625910

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1996-6-21
pubmed:abstractText
PTH-related protein (PTHrP) is the principle mediator of the syndrome of humoral hypercalcemia of malignancy and has potential paracrine actions on smooth muscle, epithelial cell growth, and placental calcium transport. The human PTHrP gene is complex: a combination of three promoters, one 5' alternative splicing event and alternative 3' splicing, which produces three PTHrP isoforms (139, 141, or 173 amino acids), results in multiple PTHrP messenger RNA (mRNA) species. We employed the RT-PCR technique to identify promoter usage and splicing patterns in a range of human cell lines. Cell line-specific utilization of the promoters and the 3' alternative splicing pathways was detected among bone, breast, kidney, and lung cell lines, although each cell line could potentially produce the three PTHrP isoforms. We also determined whether some of the known regulators of PTHrP differentially modulate promoter usage or splicing patterns. Dexamethasone decreased the abundance of each of the alternative mRNA species. In contrast, epidermal growth factor and transforming growth factor-beta treatment increased the abundance of each PTHrP mRNA species, with particularly marked effects on promoter 1- and promoter 2-initiated transcripts, especially those containing exon VII or VIII. Epidermal growth factor treatment was found to alter PTHrP splicing patterns in a manner consistent with increased transcription from promoters 1 and 2 and stabilization of exon VII- and IX-containing transcripts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
137
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1349-57
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8625910-Base Sequence, pubmed-meshheading:8625910-Cell Line, pubmed-meshheading:8625910-Cycloheximide, pubmed-meshheading:8625910-Epidermal Growth Factor, pubmed-meshheading:8625910-Genes, Regulator, pubmed-meshheading:8625910-Humans, pubmed-meshheading:8625910-Isomerism, pubmed-meshheading:8625910-Molecular Sequence Data, pubmed-meshheading:8625910-Oligonucleotide Probes, pubmed-meshheading:8625910-Parathyroid Hormone, pubmed-meshheading:8625910-Parathyroid Hormone-Related Protein, pubmed-meshheading:8625910-Polymerase Chain Reaction, pubmed-meshheading:8625910-Promoter Regions, Genetic, pubmed-meshheading:8625910-Proteins, pubmed-meshheading:8625910-RNA Splicing, pubmed-meshheading:8625910-Transcription, Genetic, pubmed-meshheading:8625910-Transforming Growth Factor beta
pubmed:year
1996
pubmed:articleTitle
Cell-specific and regulator-induced promoter usage and messenger ribonucleic acid splicing for parathyroid hormone-related protein.
pubmed:affiliation
St. Vincent's Institute of Medical Research, St. Vincent's Hospital, Victoria, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't