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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1996-6-18
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pubmed:databankReference |
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pubmed:abstractText |
An expression-cloning strategy was used to isolate a cDNA that encodes a protein that confers calcitonin gene-related peptide (CGRP) responsiveness to Xenopus laevis oocytes. A guinea pig organ of Corti (the mammalian hearing organ) cDNA library was screened by using an assay based on the cystic fibrosis transmembrane conductance regulator (CFTR). The CFTR is a chloride channel that is activated upon phosphorylation; this channel activity was used as a sensor for CGRP-induced activation of intracellular kinases. A cDNA library from guinea pig organ of Corti was screened by using this oocyte-CFTR assay. A cDNA was identified that contained an open reading frame coding for a small hydrophilic protein that is presumed to be either a CGRP receptor or a component of a CGRP receptor complex. This CGRP receptor component protein confers CGRP-specific activation to the CFTR assay, as no activation was detected upon application of calcitonin, amylin, neuropeptide Y, vasoactive intestinal peptide, or beta-endorphin. In situ hybridization demonstrated that the CGRP receptor component protein is expressed in outer hair cells of the organ of Corti and is colocalized with CGRP-containing efferent nerve terminals.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-1358871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-1646951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-1653602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-1705179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-1706537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-1715567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-1717461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-1717979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-1724683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-1762788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-2159144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-2164085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-2167177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-2231712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-2553933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-2584160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-2586755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-2786053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-2833256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-2890408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-3313277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-3322463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-3585452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-3917554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-6283379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-6302672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-6346105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-6727992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-7522902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-7592797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-7643070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-7707880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-8234011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-8293462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-8381290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-8381507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-8483499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8622957-8554605
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3455-60
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8622957-Amino Acid Sequence,
pubmed-meshheading:8622957-Animals,
pubmed-meshheading:8622957-Base Sequence,
pubmed-meshheading:8622957-Calcitonin Gene-Related Peptide,
pubmed-meshheading:8622957-Chlorides,
pubmed-meshheading:8622957-Cloning, Molecular,
pubmed-meshheading:8622957-Cystic Fibrosis Transmembrane Conductance Regulator,
pubmed-meshheading:8622957-DNA, Complementary,
pubmed-meshheading:8622957-Female,
pubmed-meshheading:8622957-Gene Expression,
pubmed-meshheading:8622957-Guinea Pigs,
pubmed-meshheading:8622957-Immunohistochemistry,
pubmed-meshheading:8622957-Molecular Sequence Data,
pubmed-meshheading:8622957-Oocytes,
pubmed-meshheading:8622957-Organ of Corti,
pubmed-meshheading:8622957-RNA, Complementary,
pubmed-meshheading:8622957-Receptors, Calcitonin Gene-Related Peptide,
pubmed-meshheading:8622957-Xenopus laevis
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pubmed:year |
1996
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pubmed:articleTitle |
Identification of a protein that confers calcitonin gene-related peptide responsiveness to oocytes by using a cystic fibrosis transmembrane conductance regulator assay.
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pubmed:affiliation |
Department of Physiology and Biophysics, Veterans Affairs Medical Center, University of Miami School of Medicine, FL 33101, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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