Linked Life Data

8607973

Source:http://linkedlifedata.com/resource/pubmed/id/8607973

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1996-5-29
pubmed:abstractText
The aims of the present series of experiments were to establish a possible relationship between bovine oocyte diameter and follicle size, investigate the developmental ability of oocytes of different diameter groups, and investigate the relationship between oocyte diameter and RNA transcriptional activity of the oocyte. Follicles were recovered from slaughterhouse ovaries by mechanical dissection, measured, and assigned to one of the following size categories: > or = 4 mm, < 3-4 mm, < 2-3 mm, < 1-2 mm, and < 1 mm. Oocytes were liberated from the follicles and their diameters recorded. The correlation coefficient between oocyte diameter and follicle size was 0.32 (P < 0.0001). Oocytes were recovered by aspiration and/or slicing of slaughterhouse ovaries and divided into four groups ( < 100 microns, 100- < 110 microns, 110- < 120 microns, and > 120 microns) based on diameter. Oocytes were processed through standard procedures for in vitro maturation and stained in order to assess nuclear development. Rates of in vitro development to metaphase II were 21.2%, 42.3%, 75.9%, and 80.7%, respectively, for the four groups. On a separate occasion immature oocytes from the above diameter groups were cultured in the presence of 3H-uridine for 45 min and scored for degree of RNA synthesis as indicated by the presence of autoradiographic labeling. Oocytes < 110 microns showed a greater degree of 3H-uridine incorporation than those > or = 110 microns, suggesting that they were involved in RNA synthesis and therefore still in the growth phase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1040-452X
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
437-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Bovine oocyte diameter in relation to maturational competence and transcriptional activity.
pubmed:affiliation
Department of Reproduction, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't