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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3-4
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pubmed:dateCreated |
1996-3-11
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pubmed:databankReference |
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pubmed:abstractText |
Equine interleukin-1 alpha (IL-1 alpha) and IL-1 beta were molecularly cloned to establish a basis for research on inflammatory and immune responses in the horse. Equine peripheral blood mononuclear cells (PBMC) were stimulated with lipopolysaccharide (LPS), and cDNA clones of equine IL-1 alpha and IL-1 beta covering the whole coding sequences were isolated from them. These equine IL-1 alpha and IL-1 beta clones contained open reading frames encoding 271 and 269 amino acids, respectively. The deduced amino acid sequence of equine IL-1 alpha showed 71.6% and 60.2% similarity with that of human and murine IL-1 alpha, respectively. Similarly, the amino acid sequence of equine IL-1 beta showed 66.7% and 61.8% similarity with that of human and murine IL-1 beta, respectively. In both equine IL-1 alpha and IL-1 beta, amino acids at miristoylation sites were well conserved. Dot blot analysis indicated that the expression of IL-1 beta was predominant to that of IL-1 alpha in equine PBMC stimulated with LPS or phorbol myristate acetate.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0165-2427
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
221-31
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8578682-Amino Acid Sequence,
pubmed-meshheading:8578682-Animals,
pubmed-meshheading:8578682-Base Sequence,
pubmed-meshheading:8578682-Carcinogens,
pubmed-meshheading:8578682-Cloning, Molecular,
pubmed-meshheading:8578682-DNA, Complementary,
pubmed-meshheading:8578682-DNA Primers,
pubmed-meshheading:8578682-Horses,
pubmed-meshheading:8578682-Humans,
pubmed-meshheading:8578682-Immunoblotting,
pubmed-meshheading:8578682-Interleukin-1,
pubmed-meshheading:8578682-Leukocytes, Mononuclear,
pubmed-meshheading:8578682-Lipopolysaccharides,
pubmed-meshheading:8578682-Lymphocyte Activation,
pubmed-meshheading:8578682-Mice,
pubmed-meshheading:8578682-Molecular Sequence Data,
pubmed-meshheading:8578682-Open Reading Frames,
pubmed-meshheading:8578682-Polymerase Chain Reaction,
pubmed-meshheading:8578682-Sequence Homology, Amino Acid,
pubmed-meshheading:8578682-Tetradecanoylphorbol Acetate
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pubmed:year |
1995
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pubmed:articleTitle |
Molecular cloning of equine interleukin-1 alpha and -beta cDNAs.
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pubmed:affiliation |
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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