Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-2-28
|
| pubmed:abstractText |
Follicular dendritic cells (FDC) are unique non-lymphoid cells found only in lymph follicles. They play a part in the survival, proliferation and differentiation of B cells. To analyse the influence of FDC on B-lymphocyte proliferation, we isolated them from human tonsils on albumin gradients and treated them with mitomycin C to prevent the multiplication of lymphoid cells harboured in their cytoplasmic evaginations. FDC cultured for 12-16 h remained attached to the substrate; non-adherent cells were carefully eliminated by washing. Purified B cells cultured alone or with contaminant-cleared FDC were maintained for 2 days in the presence or absence of various stimulants, after which tritiated thymidine uptake by these cells was measured. In the absence of activators, FDC did not induce B-cell multiplication. B cells cultured in the presence of FDC exhibited increased 3H-TdR uptake when activated with anti-CD40 MoAb, anti-immunoglobulin MoAb or transferrin, but not when stimulated with Staphylococcus aureus strain Cowan I (SAC) at a given concentration. In the latter case, B-cell proliferation clearly decreased. In control cocultures where mitomycin-C-treated non-adherent cells were used instead of FDC in the presence of the different stimulants, no increase in B-cell proliferation was observed. The results suggest that, inside the germinal centres, FDC modulation of B-cell proliferation depends on the activation state of the B cells and on the stimulant encountered.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibiotics, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogens,
http://linkedlifedata.com/resource/pubmed/chemical/Mitomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Transferrin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0300-9475
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
43
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
23-30
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8560193-Antibiotics, Antineoplastic,
pubmed-meshheading:8560193-Antibodies, Monoclonal,
pubmed-meshheading:8560193-Antigens, CD40,
pubmed-meshheading:8560193-B-Lymphocytes,
pubmed-meshheading:8560193-Cell Division,
pubmed-meshheading:8560193-Cell Separation,
pubmed-meshheading:8560193-Cells, Cultured,
pubmed-meshheading:8560193-Child,
pubmed-meshheading:8560193-Child, Preschool,
pubmed-meshheading:8560193-DNA Replication,
pubmed-meshheading:8560193-Dendritic Cells,
pubmed-meshheading:8560193-Humans,
pubmed-meshheading:8560193-Immunoglobulin G,
pubmed-meshheading:8560193-Lymphocyte Activation,
pubmed-meshheading:8560193-Mitogens,
pubmed-meshheading:8560193-Mitomycin,
pubmed-meshheading:8560193-Palatine Tonsil,
pubmed-meshheading:8560193-Staphylococcus aureus,
pubmed-meshheading:8560193-Transferrin
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
The influence of follicular dendritic cells on B-cell proliferation depends on the activation of B cells and the mitogen used.
|
| pubmed:affiliation |
Institute of Human Histology, University of Liège, Belgium.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|