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rdf:type |
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lifeskim:mentions |
umls-concept:C0008550,
umls-concept:C0016315,
umls-concept:C0021242,
umls-concept:C0036679,
umls-concept:C0041249,
umls-concept:C0237868,
umls-concept:C0439849,
umls-concept:C0445223,
umls-concept:C1511790,
umls-concept:C1552599,
umls-concept:C1704787
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pubmed:issue |
1
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pubmed:dateCreated |
1996-2-28
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pubmed:abstractText |
Micellar electrokinetic chromatography (MEKC) was applied for the separation of tryptophan and related indoles. Using a 5 mM sodium borate buffer (pH 9.2) containing 50 mM sodium dodecyl sulfate and 5% acetonitrile, eleven indoles were baseline separated in under 17 min. Most of the indoles were detected at the nM level by native fluorescence using KrF laser-induced fluorescence (LIF), which was approximately 100 times more sensitive than UV absorption detection at 200 nm. Preliminary results show that the MEKC-LIF with direct sample injection is a feasible method for assessing indole profiles in diluted urine and serum.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9673
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
718
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
203-10
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pubmed:dateRevised |
2009-1-15
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pubmed:meshHeading |
pubmed-meshheading:8556162-Electrophoresis, Capillary,
pubmed-meshheading:8556162-Fluorides,
pubmed-meshheading:8556162-Indoles,
pubmed-meshheading:8556162-Krypton,
pubmed-meshheading:8556162-Lasers,
pubmed-meshheading:8556162-Micelles,
pubmed-meshheading:8556162-Molecular Structure,
pubmed-meshheading:8556162-Sodium Dodecyl Sulfate,
pubmed-meshheading:8556162-Spectrometry, Fluorescence,
pubmed-meshheading:8556162-Tryptophan
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pubmed:year |
1995
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|
pubmed:articleTitle |
Separation of tryptophan and related indoles by micellar electrokinetic chromatography with KrF laser-induced fluorescence detection.
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pubmed:affiliation |
SAIC Frederick, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702, USA.
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pubmed:publicationType |
Journal Article
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