Linked Life Data

8537502

Source:http://linkedlifedata.com/resource/pubmed/id/8537502

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1996-2-8
pubmed:abstractText
The ability of Helicobacter pylori strains to induce interleukin-8 (IL-8) gene expression and protein secretion from gastric epithelial cell lines in vitro is variable. This cellular response is associated with bacterial expression of the CagA protein present in type I H pylori strains. To determine the role of CagA in this host cell response, an isogenic cagA negative mutant, N6.XA3, was constructed. The cagA negative isogenic mutant and the wild-type parental cagA positive strain, N6, were cocultured with AGS, ST-42 and KATO-3 gastric epithelial cell lines and secreted interleukin-8 assayed by enzyme linked immunosorbent assay. In all three cell lines there was no significant difference in the IL-8 secretion induced by the cagA negative isogenic mutant, N6.XA3, and the wild-type parent strain, N6. These studies show that CagA is not the inducer of IL-8 secretion from gastric epithelial cells. As all wild-type CagA positive strains studied to date induce IL-8, the bacterial factor(s) inducing this inflammatory response is closely associated with the expression of CagA.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8537502-1320607, http://linkedlifedata.com/resource/pubmed/commentcorrection/8537502-1677696, http://linkedlifedata.com/resource/pubmed/commentcorrection/8537502-7706517, http://linkedlifedata.com/resource/pubmed/commentcorrection/8537502-7806390, http://linkedlifedata.com/resource/pubmed/commentcorrection/8537502-7962609, http://linkedlifedata.com/resource/pubmed/commentcorrection/8537502-8096606, http://linkedlifedata.com/resource/pubmed/commentcorrection/8537502-8132812, http://linkedlifedata.com/resource/pubmed/commentcorrection/8537502-8163943, http://linkedlifedata.com/resource/pubmed/commentcorrection/8537502-8418474, http://linkedlifedata.com/resource/pubmed/commentcorrection/8537502-8478069, http://linkedlifedata.com/resource/pubmed/commentcorrection/8537502-8516329
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9746
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
967-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Induction of interleukin-8 secretion from gastric epithelial cells by a cagA negative isogenic mutant of Helicobacter pylori.
pubmed:affiliation
Division of Medicine, St James's University Hospital, Leeds.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't