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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1996-1-31
|
| pubmed:abstractText |
Gingival fibroblasts function as accessory immune cells and are capable of synthesizing cytokines in response to lipopolysaccharides (LPS) from Gram-negative microbes. Recently, we have isolated, cloned, and characterized two cell lines which exhibit characteristics of periodontal ligament (PDL) cells. In this report, we demonstrate that PDL cells showing osteoblast-like phenotype are not LPS-responsive cells. However, treatment of PDL cells with tumor necrosis factor-alpha (TNF-alpha) inhibits the expression of their osteoblast-like characteristics. As a consequence of this TNF-alpha-induced phenotypic change, PDL cells become LPS-responsive, i.e., synthesize several pro-inflammatory cytokines in response to LPS. These phenotypic changes occur at concentrations of TNF-alpha that are frequently observed in tissue exudates during periodontal inflammation, suggesting a physiological significance for these in vitro observations. It is of interest that TNF-alpha-induced phenotypic changes in PDL cells are transient, since removal of rhTNF-alpha from the supernatants of PDL cell cultures results in re-acquisition of the osteoblast-like characteristics and lack of LPS responsiveness of PDL cells. These results suggest that TNF-alpha, by regulating the PDL cell functions, may allow these cells to participate in the disease process as accessory immune cells at the expense of their structural properties.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
D
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukins,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Osteocalcin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0022-0345
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
74
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1802-11
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8530744-Alkaline Phosphatase,
pubmed-meshheading:8530744-Antigen-Presenting Cells,
pubmed-meshheading:8530744-Base Sequence,
pubmed-meshheading:8530744-Cell Line,
pubmed-meshheading:8530744-DNA Primers,
pubmed-meshheading:8530744-Fibroblasts,
pubmed-meshheading:8530744-Humans,
pubmed-meshheading:8530744-Interleukins,
pubmed-meshheading:8530744-Lipopolysaccharides,
pubmed-meshheading:8530744-Molecular Sequence Data,
pubmed-meshheading:8530744-Osteoblasts,
pubmed-meshheading:8530744-Osteocalcin,
pubmed-meshheading:8530744-Periodontal Ligament,
pubmed-meshheading:8530744-Phenotype,
pubmed-meshheading:8530744-RNA, Messenger,
pubmed-meshheading:8530744-Recombinant Proteins,
pubmed-meshheading:8530744-Time Factors,
pubmed-meshheading:8530744-Transforming Growth Factor beta,
pubmed-meshheading:8530744-Tumor Necrosis Factor-alpha
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
LPS responsiveness in periodontal ligament cells is regulated by tumor necrosis factor-alpha.
|
| pubmed:affiliation |
Division of Oral Biology, University of Pittsburgh, PA 15261, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|