Linked Life Data

8510137

Source:http://linkedlifedata.com/resource/pubmed/id/8510137

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1993-7-9
pubmed:abstractText
Iron-related tRNA alterations have been shown to occur in several pathogens but nothing has been reported about the effect of iron on meningococcal tRNA. The chromatographic elution profile of 3H-tryptophan-tRNA(trp) from a Neisseria meningitidis strain grown under different conditions was examined. The profile showed an early (P1) and a late (P2) eluting species of tRNA(trp), but the proportion of the two species varied under different growth conditions. The elution profile of trp-tRNA(trp) from bacteria grown in iron-sufficient Mueller Hinton broth yielded a minor P1 species and a major P2 species, whereas under iron-restricted growth induced by desferrioxamine, the pattern was one of a major P1 species and minor P2 species. Iron-restriction induced by human transferrin (HTF) resulted in almost equal amounts of the two tRNA(trp) species (P1 congruent to P2). Differences in the proportions of the tRNA species were also found between cells grown in liquid medium (P1 << P2) and on the same medium solidified with agar (P1 >> P2). The growth phase of the bacteria did not have any effect on the tRNA(trp) elution profile. Changes in tRNA(trp) induced by HTF were readily and completely reversible when the cells were transferred to an iron-rich medium, but those induced by desferrioxamine remained irreversible for a long period (16 h) after such transfer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-2615
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
442-8
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Alteration of transfer ribonucleic acid of Neisseria meningitidis during growth in the presence of human transferrin.
pubmed:affiliation
Microbial Pathogenicity Research Group, Clinical Research Centre, Harrow, Middlesex.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't