rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
|
pubmed:dateCreated |
1993-6-1
|
pubmed:abstractText |
CSF-1 is a dimeric peptide growth factor, stabilized by disulfide bonds. We expressed mouse CSF-1 in bacteria as a fusion protein either with glutathione S-transferase (GST) or with a six histidine tag (His-tag). Large amounts of recombinant material were obtained and purified by a single affinity chromatography step. Purified CSF-1-His-tag monomers efficiently dimerized in vitro, but the presence of variable amounts of GST-moiety in CSF-1 preparations obtained by thrombin cleavage of GST-fusion proteins (thrombin-released CSF-1) interfered with dimerization. However, the thrombin-released CSF-1 monomers possessed agonistic activity, being capable of stimulating tyrosine phosphorylation of the CSF-1 receptor and of an array of cellular proteins in living macrophages and of supporting their growth. These results show that CSF-1 dimerization is not essential for receptor activation in vivo.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0006-291X
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
30
|
pubmed:volume |
192
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
720-7
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:8484779-Animals,
pubmed-meshheading:8484779-Cell Division,
pubmed-meshheading:8484779-Cell Line,
pubmed-meshheading:8484779-Cloning, Molecular,
pubmed-meshheading:8484779-Disulfides,
pubmed-meshheading:8484779-Escherichia coli,
pubmed-meshheading:8484779-Glutathione Transferase,
pubmed-meshheading:8484779-Histidine,
pubmed-meshheading:8484779-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:8484779-Macrophages,
pubmed-meshheading:8484779-Mice,
pubmed-meshheading:8484779-Protein Folding,
pubmed-meshheading:8484779-Receptors, Platelet-Derived Growth Factor,
pubmed-meshheading:8484779-Recombinant Fusion Proteins,
pubmed-meshheading:8484779-Thrombin
|
pubmed:year |
1993
|
pubmed:articleTitle |
Bacterially expressed murine CSF-1 possesses agonistic activity in its monomeric form.
|
pubmed:affiliation |
Department of Immunbiology, Fraunhofer Institut for Toxicology and Molecular Biology, Hannover, Germany.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|