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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1993-5-25
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pubmed:abstractText |
Previously we showed that motility-related protein (MRP-1) is an antigen recognized by monoclonal antibody (mAb) M31-15 inhibiting cell motility and that the sequence of MRP-1 coincides with that of CD9. In the present study, plasmid was constructed in which human MRP-1/CD9 cDNA is expressed under the control of the Abelson murine leukemia virus promoter sequence. The expression plasmid for MRP-1/CD9 was introduced into Chinese hamster ovary cells, human lung adenocarcinoma cell line MAC10 (MRP-1 positive), and human myeloma cell line ARH77 (MRP-1 negative). All of the MRP-1/CD9 (over)expressing clones obtained from these transfected cells showed suppressed cell motility (penetration and phagokinetic track assays) depending on the degree of expression of MRP-1/CD9. Overexpression of MRP-1/CD9 by MAC10 cells resulted in the suppression of cell motility (maximally 73%) associated with considerable inhibition of the cell growth (maximally 48%). However, the inhibition of the growth of MAC10 cells by mAb M31-15 was < 17% at an antibody concentration of 1-5 micrograms/ml, which inhibits cell motility by > 90%. These results suggest that MRP-1/CD9 directly regulates cell motility and may also affect cell growth. Effects on metastasis by the expression of MRP-1 CD9 were investigated with mouse melanoma BL6 cells-BALB/c nu/nu mouse system. Metastatic potential of all transformants expressing MRP-1/CD9 was lower than that of parent BL6 cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-1559238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-1695320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-1720807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-1737392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-1856155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-2009271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-2037603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-222468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-2303480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-2324498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-2395876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-2466944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-2558106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-3017189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-3036377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-3100030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-329998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-3365686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-507192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-566614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-6306768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-6329708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-6885918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-6933469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8478605-6958929
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
177
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1231-7
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8478605-Animals,
pubmed-meshheading:8478605-Antigens, CD,
pubmed-meshheading:8478605-Antigens, CD9,
pubmed-meshheading:8478605-CHO Cells,
pubmed-meshheading:8478605-Cell Adhesion,
pubmed-meshheading:8478605-Cell Division,
pubmed-meshheading:8478605-Cell Movement,
pubmed-meshheading:8478605-Cricetinae,
pubmed-meshheading:8478605-DNA,
pubmed-meshheading:8478605-Humans,
pubmed-meshheading:8478605-Membrane Glycoproteins,
pubmed-meshheading:8478605-Mice,
pubmed-meshheading:8478605-Mice, Inbred BALB C,
pubmed-meshheading:8478605-Mice, Nude,
pubmed-meshheading:8478605-Neoplasm Metastasis,
pubmed-meshheading:8478605-Plasmids,
pubmed-meshheading:8478605-Transfection,
pubmed-meshheading:8478605-Tumor Cells, Cultured
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pubmed:year |
1993
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pubmed:articleTitle |
Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA.
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pubmed:affiliation |
Department VI, Takeda Chemical Industries, Ltd., Osaka, Japan.
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pubmed:publicationType |
Journal Article
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