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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1993-2-12
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pubmed:abstractText |
The Axd (axial defects) mutation, which causes open neural tube defects (NTD) in 25-30% of d 14 mouse embryos of heterozygous (Axd/+) matings and curly tails (CT) in 31%, can be useful in studying neurovertebral morphogenesis. Current interest focuses on the role of methionine in neurulation, because supplementation of dams with this essential amino acid (70 mg/kg body wt) has been shown to reduce by 41% the incidence of NTD among embryos of Axd/+ x Axd/+ crosses when administered on d 8 and 9 of pregnancy. The present experiments were undertaken to determine whether supplementation of dams with a higher dose of methionine could effect a greater level of remediation. At a dose of 180 mg/kg body wt, the amino acid produced a reduction in NTD of 47%, similar to that produced at 70 mg/kg body wt. Supplementation of dams of reciprocal matings of Axd/+ x BALB/cByJ or CF-1 mice allowed assessment of the effects of methionine in heterozygous embryos, which exhibit CT. The amino acid had no effect on Axd/+ embryos from the BALB crosses, but the frequency of CT declined significantly among embryos of the CF-1 outcrosses. Maternal supplements of folinic acid (33 mg/kg) or vitamin B-12 (330 mg/kg) did not alter the incidence of NTD or CT among Axd embryos. No difference in methionine concentration was detected in the serum of Axd/+ and +/+ dams.
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pubmed:commentsCorrections | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-3166
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
123
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
27-34
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8421227-Amino Acids,
pubmed-meshheading:8421227-Animals,
pubmed-meshheading:8421227-Embryo, Mammalian,
pubmed-meshheading:8421227-Female,
pubmed-meshheading:8421227-Folic Acid,
pubmed-meshheading:8421227-Injections, Intraperitoneal,
pubmed-meshheading:8421227-Male,
pubmed-meshheading:8421227-Methionine,
pubmed-meshheading:8421227-Mice,
pubmed-meshheading:8421227-Mice, Inbred BALB C,
pubmed-meshheading:8421227-Mice, Inbred Strains,
pubmed-meshheading:8421227-Mice, Mutant Strains,
pubmed-meshheading:8421227-Neural Tube Defects,
pubmed-meshheading:8421227-Vitamin B 12
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pubmed:year |
1993
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pubmed:articleTitle |
Methionine but not folinic acid or vitamin B-12 alters the frequency of neural tube defects in Axd mutant mice.
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pubmed:affiliation |
Nelson Laboratory, Rutgers University, Piscataway, NJ 08854.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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