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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1993-2-5
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pubmed:abstractText |
Enucleated mouse oocytes were successfully fertilized in vitro, and the resultant androgenetic eggs developed to the blastocyst stage. The proportion of enucleated oocytes fertilized in vitro was high (87-99%) at sperm concentrations ranging from 10-100 x 10(4)/ml. At high sperm concentrations (100-1,000 x 10(4)), 35-45% of the fertilized eggs resulted in heterozygous bispermic androgenones. The proportion of hemizygous haploid and heterozygous diploid androgenones developing to blastocysts was 11% and 43%, respectively. Hemizygous diploidization, however, showed no positive effect on development. These results clearly show that the procedure reported here is efficient and reliable for the production of androgenetic eggs.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1040-452X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
43-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8418815-Animals,
pubmed-meshheading:8418815-Diploidy,
pubmed-meshheading:8418815-Embryonic and Fetal Development,
pubmed-meshheading:8418815-Fertilization in Vitro,
pubmed-meshheading:8418815-Haploidy,
pubmed-meshheading:8418815-Mice,
pubmed-meshheading:8418815-Mice, Inbred C57BL,
pubmed-meshheading:8418815-Mice, Inbred CBA,
pubmed-meshheading:8418815-Nuclear Transfer Techniques,
pubmed-meshheading:8418815-Oocytes
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pubmed:year |
1993
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pubmed:articleTitle |
Development of androgenetic mouse embryos produced by in vitro fertilization of enucleated oocytes.
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pubmed:affiliation |
NODAI Research Institute, Tokyo University of Agriculture, Japan.
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pubmed:publicationType |
Journal Article
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