Linked Life Data

8415633

Source:http://linkedlifedata.com/resource/pubmed/id/8415633

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
1993-11-10
pubmed:abstractText
We have used the scanning tunneling microscope (STM) to image several synthetic oligonucleotides adsorbed onto a positively charged Au(111) electrode. The molecules were deposited and imaged in aqueous electrolyte under potential control, a procedure that eliminated the problem of the substrate artifacts that had limited some previous STM studies. Experiments were carried out with two types of single-stranded molecules (11 and 20 bases long) and three types of double-stranded molecules (20 and 61 base pairs and 31 bases with 25 bases paired and 6-base "sticky" ends). The molecules lie along symmetry directions on the reconstructed (23 x square root of 3) gold surface, and length measurements indicate that they adopt simple base-stacked structures. The base stacking distances are, within experimental uncertainty, equal to the 0.33 nm measured for polymeric aggregates of stacked purines by direct imaging in identical conditions. The images show features consistent with helical structures. Double helices have a major-groove periodicity that is consistent with a 36 degrees twist. The single helices appear to be more tightly twisted. A simple tunneling model of STM contrast generates good agreement between measured and calculated images.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8415633-1413243, http://linkedlifedata.com/resource/pubmed/commentcorrection/8415633-1438201, http://linkedlifedata.com/resource/pubmed/commentcorrection/8415633-1617139, http://linkedlifedata.com/resource/pubmed/commentcorrection/8415633-17780805, http://linkedlifedata.com/resource/pubmed/commentcorrection/8415633-1992517, http://linkedlifedata.com/resource/pubmed/commentcorrection/8415633-2374595, http://linkedlifedata.com/resource/pubmed/commentcorrection/8415633-2727694, http://linkedlifedata.com/resource/pubmed/commentcorrection/8415633-2911747, http://linkedlifedata.com/resource/pubmed/commentcorrection/8415633-5493278, http://linkedlifedata.com/resource/pubmed/commentcorrection/8415633-7402337, http://linkedlifedata.com/resource/pubmed/commentcorrection/8415633-8356067, http://linkedlifedata.com/resource/pubmed/commentcorrection/8415633-8422398
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
8934-8
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Structure of hydrated oligonucleotides studied by in situ scanning tunneling microscopy.
pubmed:affiliation
Department of Physics and Astronomy, Arizona State University, Tempe 85287.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't