Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1993-10-26
pubmed:abstractText
Interleukin-8 (IL-8), a chemotactic cytokine for T lymphocytes and neutrophils, is induced in several cell types by a variety of stimuli including the inflammatory cytokines IL-1 and tumor necrosis factor alpha TNF-alpha. Several cis elements, including a binding site for the inducible transcription factor NF-kappa B, have been identified in the regulatory region of the IL-8 gene. We have examined the ability of various NF-kappa B subunits to bind to, and activate transcription from, the IL-8 promoter. A nuclear complex was induced in phorbol myristate acetate-treated Jurkat T cells which bound specifically to the kappa B site of the IL-8 promoter and was inhibited by addition of purified I kappa B alpha to the reaction mixture. Only antibody to RelA (p65), but not to NFKB1 (p50), NFKB2 (p50B), c-Rel, or RelB was able to abolish binding, suggesting that RelA is a major component in these kappa B binding complexes. Gel mobility shift analysis with in vitro-translated and purified proteins indicated that whereas the kappa B element in the human immunodeficiency virus type 1 long terminal repeat bound to all members of the kappa B/Rel family examined, the IL-8 kappa B site bound only to RelA and to c-Rel and NFKB2 homodimers, but not to NFKB1 homodimers or heterodimers of NFKB1-RelA. Transient transfection analysis demonstrated a kappa B-dependent expression of the IL-8 promoter in a human fibrosarcoma cell line (8387) and in Jurkat T lymphocytes. Cotransfection with various NF-kappa B subunits indicated that RelA and c-Rel, but neither NFKB1 nor heterodimeric NFKB1-RelA, was able to activate transcription from the IL-8 promoter. Furthermore, cotransfection of NFKB1 and RelA, although able to support activation from the human immunodeficiency virus type 1 long terminal repeat, failed to activate expression from the IL-8 promoter. Antisense oligonucleotides to RelA, but not NFKB1, inhibited phorbol myristate acetate-induced IL-8 production in Jurkat T lymphocytes. These data demonstrate the differential ability of members of the kappa B/Rel family to bind to, and activate transcription from, the IL-8 promoter. Furthermore, while providing a novel example of a kappa B-regulated promoter in which the classical NF-kappa B complex is unable to activate transcription from the kappa B element, these data provide direct evidence for the role of RelA in regulation of IL-8 gene expression.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1388725, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1406630, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1472916, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1474930, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1518839, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1531086, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1542667, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1542686, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1566083, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1577270, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1577271, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1577272, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1648451, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1716285, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1718038, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1732726, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1732739, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1760839, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1856209, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1876189, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1878350, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1910690, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1935902, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1988156, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-1992489, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2001591, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2006423, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2018779, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2050119, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2115119, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2125960, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2155963, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2174352, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2181276, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2203531, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2203532, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2203533, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2204816, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2225078, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2234062, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2250017, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2478657, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2536934, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2648569, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2648570, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2663993, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2665943, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2666912, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2744487, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-2988790, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-3031512, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-3091258, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-3118464, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-6312449, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-6409419, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-6828386, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-6960240, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-8321192, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-8321203, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-8441398, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-8441404, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-8449662, http://linkedlifedata.com/resource/pubmed/commentcorrection/8413215-8497281
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6137-46
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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