Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1993-10-12
pubmed:abstractText
The translation and primary processing events of poliovirus polyproteins in HeLa cell extracts were more efficient at 34 degrees C than at 30 or 36 degrees C. The cleavage products of P2 such as 2Apro, 2BC, and 2C appeared early in the reaction before the appearance of the cleavage products of P1 and of 3CDpro, an observation suggesting that P2 was cleaved in cis by 3CDpro. Proteolytic processing of the capsid precursor P1 into VP0, VP1, and VP3 was also more efficient at 34 degrees C than at either 30 or 32 degrees C. Surprisingly, processing of 3CDpro to 3Cpro and 3Dpol was almost completely inhibited at 36 degrees C. The synthesis of virus in the cell extract was greatly enhanced at 34 degrees C over that at 30 or 32 degrees C, whereas incubation at 36 degrees C yielded very little virus. Cerulenin, an inhibitor of lipid synthesis, did not appear to affect virus-specific translation or protein processing, but it almost completely inhibited viral synthesis in vitro. Oleic acid drastically inhibited in vitro translation at 100 microM and in vitro poliovirus synthesis at 25 microM. Addition of HeLa cell smooth membranes partially restored translation but not virus formation. Our observations suggest that in vitro translation, proteolytic processing, and virus formation require intact membranes. Analysis of the in vitro translation products revealed that viral RNA polymerase activity increased linearly during incubation of the translation mixture. RNA polymerase in the crude mixture was inhibited by oleic acid but not by cerulenin. Surprisingly, oleic acid had no direct effect on oligo(U)-primed, poly(A)-dependent poly(U) synthesis catalyzed by purified 3Dpol.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-1315753, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-1329322, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-1331532, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-1656600, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-1661029, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-183011, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-1850038, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-2154600, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-2161336, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-2820130, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-2839690, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-2842953, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-2845120, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-3018300, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-3039171, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-4318974, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-6092710, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-6277514, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-6304057, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-6324172, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-6324464, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-6656641, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-8380467, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-8388831, http://linkedlifedata.com/resource/pubmed/commentcorrection/8396661-8509400
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5932-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Effects of temperature and lipophilic agents on poliovirus formation and RNA synthesis in a cell-free system.
pubmed:affiliation
Department of Microbiology, School of Medicine, State University of New York at Stony Brook 11794-5222.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.