Linked Life Data

8394946

Source:http://linkedlifedata.com/resource/pubmed/id/8394946

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1993-9-17
pubmed:abstractText
A triple wavelength spectrophotometric method was developed to monitor changes in the concentrations of oxygenated and deoxygenated hemoglobin, and in the redox state of cytochrome oxidase in living tissue. Three wavelengths of an NIR (near-infrared) laser beam were used to monitor hemoglobin (780, 805 and 830 nm) and cytochrome (cyt.) oxidase (780, 790 and 830 nm). Algorithms for calculating changes in the concentrations of oxidized cyt. oxidase (aa3), and oxygenated and deoxygenated hemoglobin were developed using NIR spectral analysis of these chromatophores. To assess the ability of these algorithms to measure independent changes in the concentration of oxy-, deoxyhemoglobin and oxidized cyt. oxidase in tissue, experiments involving a fluorocarbon (FC-43) exchange transfusion at very low hematocrits were conducted in anesthetized rats. The observed data indicate that FC-43 circulation at FiO2 1.0 can supply sufficient oxygen to the rat brain to maintain the oxidation state of mitochondrial oxidase (cyt.aa3) near pre-exchange levels. The relationships between changes in oxidized cyt. oxidase and oxyhemoglobin content in response to variations in FiO2 of inspired gas were then examined in the rat brain. These results also confirmed the ability of the triple wavelength algorithms to distinguish changes in the oxidized copper band of cyt.aa3 from those in absorption by oxy- and deoxyhemoglobin. NIR monitoring of skeletal muscle to determine the redox state of cyt. oxidase was performed in a few human cases and the results indicated that these observations can increase our understanding of the process of oxygen transport to tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0047-1828
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
817-24
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Non-invasive monitoring of the redox state of cytochrome oxidase in living tissue using near-infrared laser lights.
pubmed:affiliation
First Department of Surgery, Asahikawa Medical College, Japan.
pubmed:publicationType
Journal Article