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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-3-23
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pubmed:abstractText |
We have investigated how Drosophila P element insertions are distributed in the chromosomal region near their starting site. A single P element residing in the euchromatin of minichromosome Dp1187 was mobilized following a cross to the delta 2-3 (99B) strain, and progeny bearing transpositions were identified with a minimum of bias by performing Southern blots on progeny. Approximately 1-2% of all progeny minichromosomes contained new insertions. Many of these "local transpositions" landed very close to or within the starting P element; however, nearly 1% of all progeny chromosomes contained new insertions 1-180 kb from the donor element. More local insertions were observed in the progeny of females than from male parents, and most occurred in a preferred orientation relative to the starting element. These observations suggested that donor elements are frequently excised and reinserted locally without ever dissociating from a transposition complex. The high frequency and diverse distribution of local transpositions recovered from females suggested that the efficiency of insertional mutagenesis can be significantly enhanced by using a starting P element(s) located near the target of interest.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-1310086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-1311699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-1317315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-1325389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-1334894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-1661257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-1684332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-17246501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-17247852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-17248099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-17248592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-1849274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-1849859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-2165865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-2166229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-2177140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-2208283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-2326275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-2496301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-2556711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-2558049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-2558050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-2822534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-2835286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-2840331,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-3538420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-3917860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-6289435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-6309411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-6318121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-6326095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382178-8382177
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
133
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
361-73
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:8382178-Animals,
pubmed-meshheading:8382178-Chromosome Mapping,
pubmed-meshheading:8382178-DNA Transposable Elements,
pubmed-meshheading:8382178-Drosophila melanogaster,
pubmed-meshheading:8382178-Female,
pubmed-meshheading:8382178-Genes, Lethal,
pubmed-meshheading:8382178-Genetic Complementation Test,
pubmed-meshheading:8382178-Genetic Markers,
pubmed-meshheading:8382178-Male,
pubmed-meshheading:8382178-Mutagenesis, Insertional,
pubmed-meshheading:8382178-Recombination, Genetic,
pubmed-meshheading:8382178-Sequence Deletion,
pubmed-meshheading:8382178-Sex Characteristics
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pubmed:year |
1993
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pubmed:articleTitle |
Efficient and dispersed local P element transposition from Drosophila females.
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pubmed:affiliation |
Howard Hughes Medical Institute Research Laboratories, Carnegie Institution of Washington, Baltimore, Maryland 21210.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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