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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-3-23
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pubmed:abstractText |
Two different schemes were used to demonstrate that Drosophila P elements preferentially transpose into genomic regions close to their starting sites. A starting element with weak rosy+ marker gene expression was mobilized from its location in the subtelomeric region of the 1,300-kb Dp1187 minichromosome. Among progeny lines with altered rosy+ expression, a much higher than expected frequency contained new insertions on Dp1187. Terminal deficiencies were also recovered frequently. In a second screen, a rosy(+)-marked element causing a lethal mutation of the cactus gene was mobilized in male and female germlines, and viable revertant chromosomes were recovered that still contained a rosy+ gene due to an intrachromosomal transposition. New transpositions recovered using both methods were mapped between 0 and 128 kb from the starting site. Our results suggested that some mechanism elevates the frequency 43-67-fold with which a P element inserts near its starting site. Local transposition is likely to be useful for enhancing the rate of insertional mutation within predetermined regions of the genome.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-1310086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-1311699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-1317315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-1325389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-1334894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-1684332,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-17247852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-17248099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-1794309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-1849274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-1968635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-2123463,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-2208283,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-2509077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-2548737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-2556711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-2822534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-2827169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-2830671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-2840331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-2840332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-2841109,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8382177-826444
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
133
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pubmed:geneSymbol |
cact
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
347-59
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:8382177-Animals,
pubmed-meshheading:8382177-Base Sequence,
pubmed-meshheading:8382177-Chromosome Mapping,
pubmed-meshheading:8382177-Chromosomes,
pubmed-meshheading:8382177-DNA Transposable Elements,
pubmed-meshheading:8382177-Drosophila melanogaster,
pubmed-meshheading:8382177-Female,
pubmed-meshheading:8382177-Genes, Lethal,
pubmed-meshheading:8382177-In Situ Hybridization,
pubmed-meshheading:8382177-Male,
pubmed-meshheading:8382177-Molecular Sequence Data,
pubmed-meshheading:8382177-Mutagenesis, Insertional,
pubmed-meshheading:8382177-Recombination, Genetic,
pubmed-meshheading:8382177-Sequence Deletion
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pubmed:year |
1993
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pubmed:articleTitle |
Preferential transposition of Drosophila P elements to nearby chromosomal sites.
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pubmed:affiliation |
Howard Hughes Medical Institute Research Laboratories, Carnegie Institution of Washington, Baltimore, Maryland 21210.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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