Linked Life Data

8360077

Source:http://linkedlifedata.com/resource/pubmed/id/8360077

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1993-9-24
pubmed:abstractText
Esterase 6 in Drosophila melanogaster, Drosophila simulans and Drosophila mauritiana is produced in several life stages and diverse tissues, but the major pulse of expression is in the sperm ejaculatory duct of adult males. Comparison of EST6 activity levels among several lines of D. melanogaster, D. simulans and D. mauritiana reveals two major quantitative differences among the species. First, newly eclosed females of both D. simulans and D. mauritiana show significantly higher EST6 activity than those of D. melanogaster. Secondly, 5-day-old adult D. simulans have significantly higher activities than D. mauritiana in both sexes and significantly higher activity than D. melanogaster in males. The genetic bases of the differences between D. melanogaster and the other species are investigated through germ line transfer of the D. simulans and D. mauritiana Est-6 genes plus 1.2 kb of 5' and 0.2 kb of their 3' flanking sequences into D. melanogaster. The newly eclosed female activities of the transformants resemble those of the two donor species, suggesting that the interspecific differences in this aspect of expression are due to cis-inherited factors contained within the transferred DNA. In contrast, the 5-day adult activity of the D. simulans transgene resembles the recipient species, D. melanogaster, suggesting that the difference between D. simulans and D. melanogaster in this aspect of expression is due to trans-acting factors. We also find that third instar larval activities of the D. simulans transgene and 5-day male activities of the D. mauritiana transgene are lower than those of either parental species, suggesting that not all the promoter elements relevant to these aspects of expression are included in the transferred DNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0018-067X
pubmed:author
pubmed:issnType
Print
pubmed:volume
71 ( Pt 1)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
41-50
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Regulatory aspects of esterase 6 activity variation in sibling Drosophila species.
pubmed:affiliation
CSIRO Division of Entomology, Canberra, Australia.
pubmed:publicationType
Journal Article