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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1993-9-14
|
| pubmed:abstractText |
In order to establish the pathophysiological roles of IL-8, rabbit IL-8 was expressed in Escherichia coli and purified to homogeneity by sequential chromatography on heparin agarose, CM-HPLC, and RP-HPLC. The purified recombinant rabbit IL-8 was homogeneous on SDS-PAGE and the ED50 of neutrophil chemotactic activity for rabbit peritoneal neutrophils was 2 ng/ml. The binding of 125I-labeled rabbit IL-8 to rabbit neutrophils was inhibited by unlabeled human IL-8 as well as rabbit IL-8 but not by another leucocyte chemotactic cytokine (chemokine), monocyte chemotactic and activating factor. Scatchard plot analysis of the binding of 125I-labeled rabbit IL-8 to rabbit peritoneal neutrophils revealed that the rabbit neutrophils have two affinity classes of receptors for IL-8 (Kd = 2.3 nM, 4.1 x 10(4) sites/cell; Kd = 18.0 nM, 11.4 x 10(4) sites/cell). It was found that a previously generated mouse anti-human IL-8 mAb, WS-4, inhibited the binding of 125I-labeled rabbit IL-8 to rabbit neutrophils, and blocked neutrophil chemotaxis in vitro in a specific and dose-dependent manner. An ELISA system for rabbit IL-8 was established using this mAb and guinea pig polyclonal antibodies to recombinant rabbit IL-8 to measure the levels of IL-8 in rabbit plasma. Intravenous administration of lipopolysaccharide (LPS) (100 micrograms) in rabbits caused the highest level of IL-8 in blood at around 2 h. Intravenous administration of WS-4 (10 mg) inhibited neutrophil infiltration at the site of LPS injection into the rabbit skin, suggesting that IL-8 is essential in the recruitment of neutrophils at sites of acute inflammation in vivo.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0953-8178
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:geneSymbol |
IL-8
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
681-90
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8347559-Amino Acid Sequence,
pubmed-meshheading:8347559-Animals,
pubmed-meshheading:8347559-Antibodies, Monoclonal,
pubmed-meshheading:8347559-Base Sequence,
pubmed-meshheading:8347559-Chemotaxis, Leukocyte,
pubmed-meshheading:8347559-Cloning, Molecular,
pubmed-meshheading:8347559-Cross Reactions,
pubmed-meshheading:8347559-DNA,
pubmed-meshheading:8347559-Dermatitis,
pubmed-meshheading:8347559-Escherichia coli,
pubmed-meshheading:8347559-Female,
pubmed-meshheading:8347559-Gene Expression,
pubmed-meshheading:8347559-Humans,
pubmed-meshheading:8347559-Interleukin-8,
pubmed-meshheading:8347559-Lipopolysaccharides,
pubmed-meshheading:8347559-Molecular Sequence Data,
pubmed-meshheading:8347559-Neutrophils,
pubmed-meshheading:8347559-Rabbits,
pubmed-meshheading:8347559-Recombinant Proteins
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Expression of recombinant rabbit IL-8 in Escherichia coli and establishment of the essential involvement of IL-8 in recruiting neutrophils into lipopolysaccharide-induced inflammatory site of rabbit skin.
|
| pubmed:affiliation |
Department of Hygiene, Kanazawa University, Japan.
|
| pubmed:publicationType |
Journal Article
|