Linked Life Data

8333862

Source:http://linkedlifedata.com/resource/pubmed/id/8333862

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-8-17
pubmed:abstractText
A method is presented for the rapid extraction of proteins from Drosophila tissues. This method involves lysis of embryos in high concentrations of guanidine hydrochloride, followed by ultracentrifugation in a guanidine hydrochloride step gradient. Several membrane-associated antigens, including Notch and the beta subunit of PS integrin are enriched in this preparation. The quantity of the proteoglycan, DROP-1, obtained from Drosophila eggs and testes was also greatly improved by the guanidine hydrochloride extraction method. This method should prove useful in the isolation and characterization of many Drosophila antigens, particularly those associated with cell membranes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
194
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
490-5
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Detection of electrophoretic variants of Notch, PS integrin, and DROP-1 proteins in Drosophila following extraction in guanidine hydrochloride.
pubmed:affiliation
Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't