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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1993-8-5
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pubmed:abstractText |
The NH2-terminal amino sequence through the first 20 amino acids was obtained for transferrin-binding protein (TBP)1 from three strains of Neisseria meningitidis. These were identical except for a glutamine to a glycine substitution at residue 6 in one case. The sequences of the NH2-terminal 20 amino acids of TBP2 from the same three strains were also determined; one TBP2 had a M(r) of 68,000 and the other two of 78,000. Sequences were identical up to residue 13 in all three proteins. Peptides based on the NH2-terminal sequences of TBP1 and 2 were synthesized, linked to Keyhole Limpet haemocyanin and used to raise antibodies in rabbits. Anti-peptide antibodies cross-reacted on immunoblotting with the respective TBPs from all meningococcal strains tested, as well as with those from N. gonorrhoeae, suggesting that the NH2-terminals of these proteins are well conserved in the Neisseria. Neither anti-peptide serum reacted with the analogous TBP1 and 2 from Haemophilus influenzae, although common epitopes have previously been shown to exist.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0378-1097
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
109
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
85-91
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8319886-Amino Acid Sequence,
pubmed-meshheading:8319886-Animals,
pubmed-meshheading:8319886-Antibodies, Bacterial,
pubmed-meshheading:8319886-Antigens, Bacterial,
pubmed-meshheading:8319886-Bacterial Proteins,
pubmed-meshheading:8319886-Carrier Proteins,
pubmed-meshheading:8319886-Conserved Sequence,
pubmed-meshheading:8319886-Cross Reactions,
pubmed-meshheading:8319886-Haemophilus influenzae,
pubmed-meshheading:8319886-Iron-Binding Proteins,
pubmed-meshheading:8319886-Molecular Sequence Data,
pubmed-meshheading:8319886-Neisseria gonorrhoeae,
pubmed-meshheading:8319886-Neisseria meningitidis,
pubmed-meshheading:8319886-Peptide Fragments,
pubmed-meshheading:8319886-Rabbits,
pubmed-meshheading:8319886-Sequence Homology, Amino Acid,
pubmed-meshheading:8319886-Species Specificity,
pubmed-meshheading:8319886-Transferrin,
pubmed-meshheading:8319886-Transferrin-Binding Proteins
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pubmed:year |
1993
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pubmed:articleTitle |
Antigenic relationships of transferrin-binding proteins from Neisseria meningitidis, N. gonorrhoeae and Haemophilus influenzae: cross-reactivity of antibodies to NH2-terminal peptides.
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pubmed:affiliation |
National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, UK.
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pubmed:publicationType |
Journal Article,
Comparative Study
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