| pubmed-article:8317549 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8317549 | lifeskim:mentions | umls-concept:C0040300 | lld:lifeskim |
| pubmed-article:8317549 | lifeskim:mentions | umls-concept:C0032659 | lld:lifeskim |
| pubmed-article:8317549 | lifeskim:mentions | umls-concept:C0004561 | lld:lifeskim |
| pubmed-article:8317549 | lifeskim:mentions | umls-concept:C1704387 | lld:lifeskim |
| pubmed-article:8317549 | lifeskim:mentions | umls-concept:C1522642 | lld:lifeskim |
| pubmed-article:8317549 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:8317549 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:8317549 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8317549 | pubmed:dateCreated | 1993-7-29 | lld:pubmed |
| pubmed-article:8317549 | pubmed:abstractText | A method was established to detect clonal B cell populations in frozen and paraffin-embedded tissues. The method is based on the polymerase chain reaction amplification of rearranged VH and V kappa genes using V gene family-specific primers. Monoclonal B cell populations could be detected in peripheral blood lymphocyte DNA in all 16 cases of B-CLL and immunocytoma investigated and in 8 of 10 cases of B cell non-Hodgkin's lymphoma using frozen and paraffin sections. The amplification of V kappa rearrangements in addition to the VH amplification is a useful tool to verify the results of the heavy chain rearrangement and to detect proliferation of a B cell clone in cases in which no VH product was obtained. In spite of the degradation of DNA in paraffin-embedded tissues, we were able to find amplified polymerase chain reaction products of about 350 bp length in 8 of 10 cases analyzed. The method presented here may be helpful in routine diagnosis of B cell non-Hodgkin's lymphoma using frozen or paraffin-embedded specimens. | lld:pubmed |
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| pubmed-article:8317549 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8317549 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8317549 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:8317549 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8317549 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:8317549 | pubmed:issn | 0002-9440 | lld:pubmed |
| pubmed-article:8317549 | pubmed:author | pubmed-author:RajewskyKK | lld:pubmed |
| pubmed-article:8317549 | pubmed:author | pubmed-author:ZhaoMM | lld:pubmed |
| pubmed-article:8317549 | pubmed:author | pubmed-author:KüppersRR | lld:pubmed |
| pubmed-article:8317549 | pubmed:author | pubmed-author:HansmannM LML | lld:pubmed |
| pubmed-article:8317549 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8317549 | pubmed:volume | 143 | lld:pubmed |
| pubmed-article:8317549 | pubmed:geneSymbol | V<down>H</down> | lld:pubmed |
| pubmed-article:8317549 | pubmed:geneSymbol | D<down>H</down> | lld:pubmed |
| pubmed-article:8317549 | pubmed:geneSymbol | V<down>&kgr;</down> | lld:pubmed |
| pubmed-article:8317549 | pubmed:geneSymbol | J<down>&kgr;</down> | lld:pubmed |
| pubmed-article:8317549 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8317549 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8317549 | pubmed:pagination | 230-9 | lld:pubmed |
| pubmed-article:8317549 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8317549 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8317549 | pubmed:articleTitle | Detection of clonal B cell populations in paraffin-embedded tissues by polymerase chain reaction. | lld:pubmed |
| pubmed-article:8317549 | pubmed:affiliation | I. Medizinische Universitätsklinik Köln, Germany. | lld:pubmed |
| pubmed-article:8317549 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8317549 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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