|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1993-7-29
|
|
pubmed:abstractText |
A method was established to detect clonal B cell populations in frozen and paraffin-embedded tissues. The method is based on the polymerase chain reaction amplification of rearranged VH and V kappa genes using V gene family-specific primers. Monoclonal B cell populations could be detected in peripheral blood lymphocyte DNA in all 16 cases of B-CLL and immunocytoma investigated and in 8 of 10 cases of B cell non-Hodgkin's lymphoma using frozen and paraffin sections. The amplification of V kappa rearrangements in addition to the VH amplification is a useful tool to verify the results of the heavy chain rearrangement and to detect proliferation of a B cell clone in cases in which no VH product was obtained. In spite of the degradation of DNA in paraffin-embedded tissues, we were able to find amplified polymerase chain reaction products of about 350 bp length in 8 of 10 cases analyzed. The method presented here may be helpful in routine diagnosis of B cell non-Hodgkin's lymphoma using frozen or paraffin-embedded specimens.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-1648975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-1649888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-1699122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-1700749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-1704265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-1713635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-1899102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-1908883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-1909411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-1976522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-2012750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-2019291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-2115572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-2124587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-2370312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-2448875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-3030728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-3079640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-3109221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-3123998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-3126251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-3127527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-3141924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-3243276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-3396540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-378907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-6101209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-6198355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-6276389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-6300689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8317549-6783958
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0002-9440
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
143
|
|
pubmed:geneSymbol |
D<down>H</down>,
J<down>&kgr;</down>,
V<down>&kgr;</down>,
V<down>H</down>
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
230-9
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:8317549-B-Lymphocyte Subsets,
pubmed-meshheading:8317549-Base Sequence,
pubmed-meshheading:8317549-Blotting, Southern,
pubmed-meshheading:8317549-Clone Cells,
pubmed-meshheading:8317549-Electrophoresis, Agar Gel,
pubmed-meshheading:8317549-Frozen Sections,
pubmed-meshheading:8317549-Gene Amplification,
pubmed-meshheading:8317549-Gene Rearrangement, B-Lymphocyte, Heavy Chain,
pubmed-meshheading:8317549-Gene Rearrangement, B-Lymphocyte, Light Chain,
pubmed-meshheading:8317549-Humans,
pubmed-meshheading:8317549-Immunoglobulin Heavy Chains,
pubmed-meshheading:8317549-Immunoglobulin Light Chains,
pubmed-meshheading:8317549-Leukemia, B-Cell,
pubmed-meshheading:8317549-Molecular Sequence Data,
pubmed-meshheading:8317549-Paraffin Embedding,
pubmed-meshheading:8317549-Polymerase Chain Reaction
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
Detection of clonal B cell populations in paraffin-embedded tissues by polymerase chain reaction.
|
|
pubmed:affiliation |
I. Medizinische Universitätsklinik Köln, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
