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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1994-3-9
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pubmed:databankReference |
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pubmed:abstractText |
The let-60 ras gene of Caenorhabditis elegans is one of the key players in a signal transduction pathway that controls the choice between vulval and epidermal differentiation in response to extracellular signals. To identify components acting downstream of let-60 ras in the vulval signaling pathway, we have identified a reduction-of-function mutation in the sur-1 gene that completely suppresses the multivulva phenotype of a hyperactive let-60 ras mutation. About 10% of animals homozygous for the sur-1 mutation also display a specific and intriguing vulval cell lineage defect. In addition, the sur-1 mutation results in a cold-sensitive egg-laying defective phenotype and a partial larval lethal phenotype. We have cloned the sur-1 gene by DNA-mediated transformation and have shown that it encodes a protein similar in overall structure to mammalian MAP kinases (ERKs). The functional homology between Sur-1 MAP kinase and mammalian MAP kinases was also demonstrated by the ability of a rat ERK2 kinase to rescue the sur-1 mutant phenotypes. Genetic double-mutant analyses place sur-1 downstream of let-60 ras but upstream of lin-1 in the vulval signaling pathway. Our results provide further evidence for the extreme conservation of Ras-mediated signaling pathway between worms and humans and for the function of MAP kinases in cell signaling processes that control cell differentiation and animal development.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Helminth Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Multidrug Resistance-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, Inwardly...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Drug,
http://linkedlifedata.com/resource/pubmed/chemical/let-60 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/ras Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/sulfonylurea receptor
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0890-9369
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:geneSymbol |
let-60 ras,
sur-1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
147-59
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8299935-ATP-Binding Cassette Transporters,
pubmed-meshheading:8299935-Amino Acid Sequence,
pubmed-meshheading:8299935-Animals,
pubmed-meshheading:8299935-Base Sequence,
pubmed-meshheading:8299935-Caenorhabditis elegans,
pubmed-meshheading:8299935-Caenorhabditis elegans Proteins,
pubmed-meshheading:8299935-Cell Differentiation,
pubmed-meshheading:8299935-Cloning, Molecular,
pubmed-meshheading:8299935-DNA, Complementary,
pubmed-meshheading:8299935-Embryonic Induction,
pubmed-meshheading:8299935-Female,
pubmed-meshheading:8299935-Genes, ras,
pubmed-meshheading:8299935-Helminth Proteins,
pubmed-meshheading:8299935-Humans,
pubmed-meshheading:8299935-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:8299935-Molecular Sequence Data,
pubmed-meshheading:8299935-Multidrug Resistance-Associated Proteins,
pubmed-meshheading:8299935-Phenotype,
pubmed-meshheading:8299935-Potassium Channels, Inwardly Rectifying,
pubmed-meshheading:8299935-Protein-Serine-Threonine Kinases,
pubmed-meshheading:8299935-Protein-Tyrosine Kinases,
pubmed-meshheading:8299935-Receptors, Drug,
pubmed-meshheading:8299935-Sequence Homology, Amino Acid,
pubmed-meshheading:8299935-Signal Transduction,
pubmed-meshheading:8299935-Suppression, Genetic,
pubmed-meshheading:8299935-Vulva,
pubmed-meshheading:8299935-ras Proteins
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pubmed:year |
1994
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pubmed:articleTitle |
Suppression of activated Let-60 ras protein defines a role of Caenorhabditis elegans Sur-1 MAP kinase in vulval differentiation.
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pubmed:affiliation |
Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder 80309-0347.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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