Linked Life Data

8263204

Source:http://linkedlifedata.com/resource/pubmed/id/8263204

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1994-1-27
pubmed:abstractText
Stool specimens obtained from 123 hospitalized patients with acute secretory diarrhea admitted to the Infectious Diseases Hospital, Calcutta, India, were examined for isolation of Vibrio cholerae O1 by direct or enrichment plating on selective media for cholera toxin (CT) by bead enzyme-linked immunosorbent assay (bead-ELISA) and for the CT gene by polymerase chain reaction (PCR). V. cholerae O1 was isolated either by direct culture or by enrichment culture from 70 stool specimens, all of which gave positive results by PCR. Eleven specimens which were culture negative and bead-ELISA positive also gave positive results by PCR. In addition, 13 more specimens which were negative by both the culture method and bead-ELISA, were positive by PCR. With the combined results of both the culture method and the CT bead-ELISA, a confirmed laboratory diagnosis of cholera could be made from 81 stool specimens, while the combined results of the three methods, including PCR, yielded a positive result for 94 specimens examined. From these data, we conclude that PCR provides a more sensitive and specific assay for rapid diagnosis of cholera than currently available methods.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8263204-1584072, http://linkedlifedata.com/resource/pubmed/commentcorrection/8263204-1629335, http://linkedlifedata.com/resource/pubmed/commentcorrection/8263204-1629337, http://linkedlifedata.com/resource/pubmed/commentcorrection/8263204-1673518, http://linkedlifedata.com/resource/pubmed/commentcorrection/8263204-1696283, http://linkedlifedata.com/resource/pubmed/commentcorrection/8263204-1774258, http://linkedlifedata.com/resource/pubmed/commentcorrection/8263204-1975354, http://linkedlifedata.com/resource/pubmed/commentcorrection/8263204-3290242, http://linkedlifedata.com/resource/pubmed/commentcorrection/8263204-6343419, http://linkedlifedata.com/resource/pubmed/commentcorrection/8263204-6355159, http://linkedlifedata.com/resource/pubmed/commentcorrection/8263204-6995331
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
3068-70
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Detection of cholera toxin gene in stool specimens by polymerase chain reaction: comparison with bead enzyme-linked immunosorbent assay and culture method for laboratory diagnosis of cholera.
pubmed:affiliation
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't