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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1994-1-10
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pubmed:abstractText |
13C double-quantum filtered correlation spectroscopy (DQF-COSY) provides a novel method for the detection of reactions involving carbon-bond scissions. We report the use of this technique to investigate isotopic exchange reactions of the non-oxidative pentose phosphate pathway in human erythrocytes. These exchange reactions resulted in the formation of a range of isotopic isomers (isotopomers) of glucose 6-phosphate after incubation of a mixture of universally 13C-labelled and unlabelled glucose 6-phosphate with fructose 1,6-bisphosphate and haemolysates. These isotopomers were detected in the coupling patterns of cross-peaks within the DQF-COSY spectrum of the deproteinized sample. A computer model which fully describes the reactions of the non-oxidative pentose phosphate pathway in human erythrocytes has previously been constructed and tested with 31P n.m.r. time-course data in our laboratory. This model was refined using 13C n.m.r. time-course data and extended to include the range of isotopomers which may be formed experimentally by the reactions of the non-oxidative pentose phosphate pathway. The isotopomer ratios obtained experimentally from the DQF-COSY spectrum were consistent with simulations generated by this model.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8257428-13762870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8257428-14212152,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8257428-1965276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8257428-1975735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8257428-2053137,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8257428-2194483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8257428-2371280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8257428-2734314,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8257428-2924774,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8257428-3155692,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8257428-7470039
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
296 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
379-87
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8257428-Carbon Isotopes,
pubmed-meshheading:8257428-Computer Simulation,
pubmed-meshheading:8257428-Erythrocytes,
pubmed-meshheading:8257428-Glucose-6-Phosphate,
pubmed-meshheading:8257428-Glucosephosphates,
pubmed-meshheading:8257428-Humans,
pubmed-meshheading:8257428-Isomerism,
pubmed-meshheading:8257428-Kinetics,
pubmed-meshheading:8257428-Magnetic Resonance Spectroscopy,
pubmed-meshheading:8257428-Mathematics,
pubmed-meshheading:8257428-Models, Biological,
pubmed-meshheading:8257428-Pentose Phosphate Pathway,
pubmed-meshheading:8257428-Time Factors
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pubmed:year |
1993
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pubmed:articleTitle |
13C n.m.r. isotopomer and computer-simulation studies of the non-oxidative pentose phosphate pathway of human erythrocytes.
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pubmed:affiliation |
Department of Biochemistry, University of Sydney, NSW, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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