Linked Life Data

8253282

Source:http://linkedlifedata.com/resource/pubmed/id/8253282

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-1-7
pubmed:abstractText
Gene products present in mouse oocytes direct development until the two-cell stage and may be important in later development. Here, we demonstrate that expression of a specific maternal protein can be disrupted in mouse oocytes using transgenic antisense RNA technology. An oocyte-specific promoter (mZP3) was utilized to express antisense RNA directed against maternal mRNA encoding tissue-type plasminogen activator (tPA). Antisense expression results in reduced levels of tPA mRNA and enzyme activity in mouse oocytes. We also provide evidence for a novel mechanism of antisense-mediated translational inhibition, whereby the cytoplasmic polyadenylation of maternal tPA mRNA is altered. This strategy should prove applicable to functional studies of other murine maternal mRNAs in an in vivo environment.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:volume
160
pubmed:geneSymbol
ZAPt, mZP3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
543-53
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Creating maternal effect mutations in transgenic mice: antisense inhibition of an oocyte gene product.
pubmed:affiliation
Department of Pharmacology, State University of New York at Stony Brook 11794-8651.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.