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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1993-12-16
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pubmed:databankReference |
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pubmed:abstractText |
Chromosomal gene aac(6')-Ii of Enterococcus faecium CIP 54-32, encoding a 6'-N-aminoglycoside acetyltransferase was characterized. The gene was identified as a coding sequence of 549 bp corresponding to a protein with a calculated mass of 20,666 Da. Analysis of the sequence of the deduced protein suggested that it was the second member of a subfamily of AAC(6')-I enzymes. Insertional inactivation of aac(6')-Ii led to aminoglycoside susceptibility of CIP 54-32, suggesting that this gene plays a role in resistance to AAC(6')-I substrates. The gene was detected by DNA hybridization in all 26 strains of E. faecium tested but not in 44 other enterococci of 13 species. These data suggest that the aac(6')-Ii gene is species specific and may be used to identify E. faecium.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8239603-113469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8239603-1354954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8239603-1577689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8239603-1657722,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8239603-8385262
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0066-4804
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:geneSymbol |
aac(6')-Ii
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1896-903
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8239603-Acetyltransferases,
pubmed-meshheading:8239603-Aminoglycosides,
pubmed-meshheading:8239603-Anti-Bacterial Agents,
pubmed-meshheading:8239603-Bacterial Proteins,
pubmed-meshheading:8239603-Base Sequence,
pubmed-meshheading:8239603-Blotting, Southern,
pubmed-meshheading:8239603-Chromosomes, Bacterial,
pubmed-meshheading:8239603-Cloning, Molecular,
pubmed-meshheading:8239603-DNA, Bacterial,
pubmed-meshheading:8239603-DNA Transposable Elements,
pubmed-meshheading:8239603-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8239603-Enterococcus faecium,
pubmed-meshheading:8239603-Escherichia coli,
pubmed-meshheading:8239603-Molecular Sequence Data,
pubmed-meshheading:8239603-Nucleic Acid Hybridization,
pubmed-meshheading:8239603-Plasmids
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pubmed:year |
1993
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pubmed:articleTitle |
Characterization of the chromosomal aac(6')-Ii gene specific for Enterococcus faecium.
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pubmed:affiliation |
Service de Bactériologie-Virologie-Hygiène, Hôpital de Bicêtre, Université Paris Sud, France.
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pubmed:publicationType |
Journal Article
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