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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1993-12-6
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pubmed:abstractText |
Interleukin-1, a soluble polypeptide, plays an important role in inflammatory reactions by increasing prostaglandin E2 (PGE2) generation. Human recombinant IL-1 receptor antagonist (hrIL-1ra) is a natural inhibitor of IL-1 which blocks its activity in several inflammatory states. In these studies we found that hrIL-1ra (250 mg/ml) inhibits the generation of PGE2, as measured by RIA method, in minced mouse granuloma tissue (700 mg) treated overnight with LPS (10-1000 ng/ml) or hrIL-1 beta (0.1-10 ng/ml). In addition, we show that hrIL-1ra (250 ng/ml) strongly inhibited IL-1 alpha and IL-1 beta, as measured by ELISA method, in the minced granuloma tissue treated overnight with LPS 1 micrograms/ml or IL-1 beta (10 ng/ml). The granuloma tissue induced in mice by a dorsal subcutaneous injection (0.2 ml) of a saturated solution (1:40 dilution) of KMnO4 crystals, presented an alkaline phosphatase activity which was not inhibited by two intraperitoneal administrations of hrIL-1ra 20 micrograms/200 ml bolus injections (given at the same time as KMnO4 injection and one 24 h later). These results show for the first time that hrIL-1ra blocks PGE2, IL-1 alpha and IL-1 beta but not alkaline phosphatase activity, which is a marker in growing bone and in calcific and inflamed tissue.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/IL1RN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Il1rn protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin 1 Receptor Antagonist...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Permanganate,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0165-2478
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8225402-Alkaline Phosphatase,
pubmed-meshheading:8225402-Animals,
pubmed-meshheading:8225402-Chronic Disease,
pubmed-meshheading:8225402-Dinoprostone,
pubmed-meshheading:8225402-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8225402-Granuloma,
pubmed-meshheading:8225402-Interleukin 1 Receptor Antagonist Protein,
pubmed-meshheading:8225402-Interleukin-1,
pubmed-meshheading:8225402-Lipopolysaccharides,
pubmed-meshheading:8225402-Male,
pubmed-meshheading:8225402-Mice,
pubmed-meshheading:8225402-Potassium Permanganate,
pubmed-meshheading:8225402-Radioimmunoassay,
pubmed-meshheading:8225402-Recombinant Proteins,
pubmed-meshheading:8225402-Sialoglycoproteins,
pubmed-meshheading:8225402-Skin Diseases
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pubmed:year |
1993
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pubmed:articleTitle |
Human recombinant interleukin-1 receptor antagonist (hrIL-1RA) inhibits prostaglandin E2 (PGE2) generation but not alkaline phosphatase activity in in vivo chronic granulomatous tissue induced by KMnO4.
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pubmed:affiliation |
Immunology Division, Institute of Experimental Medicine, University of Chieti Medical School, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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