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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1993-12-21
|
| pubmed:abstractText |
There were dramatic species differences in the level of [125I]recombinant human interleukin-1 alpha ([125I]hIL-1 alpha) binding with high levels of binding present in mouse and rabbit tissues, while no specific binding was present in rat and guinea pig tissues. Utilizing [125I]hIL-1 alpha, moderate to high levels of specific binding were observed in EL-4 6.1 cells (representative of Type I IL-1 receptors) and in mouse hippocampus, spleen and testis; however, no specific [125I]hIL-1 alpha binding was present in Raji cells (representative of Type II IL-1 receptors) and in rat tissues. On the other hand, utilizing [125I]hIL-1 beta, high specific IL-1 binding was present in EL-4 6.1 and Raji cells and moderate binding was evident in mouse tissues, whereas specific [125I]hIL-1 beta binding to rat tissues was not detectable. Moreover, no IL-1 binding in rat tissues was observed using [125I]hIL-1 receptor antagonist, [125I]mouse IL-1 beta or the homologous radioligands [125I]rat IL-1 beta or [125I]rat IL-1 receptor antagonist. These data demonstrate that under optimal conditions for labeling Type I or Type II IL-1 receptors, no specific binding is observed in rat tissues suggesting the presence of novel IL-1 receptor(s) in rat tissues.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0006-8993
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
24
|
| pubmed:volume |
623
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
172-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8221088-Animals,
pubmed-meshheading:8221088-Brain,
pubmed-meshheading:8221088-Cell Membrane,
pubmed-meshheading:8221088-Guinea Pigs,
pubmed-meshheading:8221088-Humans,
pubmed-meshheading:8221088-Interleukin-1,
pubmed-meshheading:8221088-Iodine Radioisotopes,
pubmed-meshheading:8221088-Ligands,
pubmed-meshheading:8221088-Male,
pubmed-meshheading:8221088-Mice,
pubmed-meshheading:8221088-Mice, Inbred C57BL,
pubmed-meshheading:8221088-Organ Specificity,
pubmed-meshheading:8221088-Rabbits,
pubmed-meshheading:8221088-Rats,
pubmed-meshheading:8221088-Receptors, Interleukin-1,
pubmed-meshheading:8221088-Recombinant Proteins,
pubmed-meshheading:8221088-Species Specificity,
pubmed-meshheading:8221088-Spleen,
pubmed-meshheading:8221088-Testis,
pubmed-meshheading:8221088-Tumor Cells, Cultured
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Species differences in [125I]interleukin-1 binding in brain, endocrine and immune tissues.
|
| pubmed:affiliation |
Central Nervous System Diseases Research, DuPont Merck Pharmaceutical Co., Wilmington, DE 19880-0400.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|