| pubmed-article:8218933 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8218933 | lifeskim:mentions | umls-concept:C1123019 | lld:lifeskim |
| pubmed-article:8218933 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:8218933 | lifeskim:mentions | umls-concept:C0037783 | lld:lifeskim |
| pubmed-article:8218933 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:8218933 | lifeskim:mentions | umls-concept:C2612728 | lld:lifeskim |
| pubmed-article:8218933 | lifeskim:mentions | umls-concept:C1511636 | lld:lifeskim |
| pubmed-article:8218933 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:8218933 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:8218933 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:8218933 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8218933 | pubmed:dateCreated | 1993-12-15 | lld:pubmed |
| pubmed-article:8218933 | pubmed:abstractText | We have expressed and partially purified recombinant ovine tumour necrosis factor alpha (rovTNF-alpha) using a yeast Ty, virus like particle, expression system. RovTNF-alpha is at least as active as recombinant human TNF-alpha (rhTNF-alpha) in two different bio-assays performed on ovine material, whilst approximately 1000-fold more rovrTNF-alpha than rhTNF-alpha is required to induce the same level of cytotoxicity in TNF-sensitive murine cell lines L929 and WEHI 164 clone 13. When cytotoxic assays are performed on the porcine TNF sensitive cell line PK(15)-1512, rovTNF-alpha shows about 2 logs greater activity than on murine cells, whilst rhTNF-alpha is about 1 log more active. A monoclonal antibody, raised against rovTNF-alpha, has been used to demonstrate the presence of nanogram amounts of an appropriately sized glycoprotein to be native ovine TNF-alpha in supernants of LPS stimulated ovine alveolar macrophages. These samples show no detectable cytotoxicity to L929 cells, although they show activity attributable to TNF-alpha (through neutralization by a polyclonal antiserum raised to rovTNF-alpha) in an assay on ovine material. The relative lack of activity on murine cells helps to explain previous reports of inability to assay native ovine TNF-alpha using these cells, in spite of their routine use to assay TNF-alpha from several other species. The sequence features in ovine TNF-alpha which might reduce its affinity for the murine TNF type 1 receptor are discussed. | lld:pubmed |
| pubmed-article:8218933 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8218933 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8218933 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8218933 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8218933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8218933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8218933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8218933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8218933 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8218933 | pubmed:month | May | lld:pubmed |
| pubmed-article:8218933 | pubmed:issn | 1043-4666 | lld:pubmed |
| pubmed-article:8218933 | pubmed:author | pubmed-author:BertoneCC | lld:pubmed |
| pubmed-article:8218933 | pubmed:author | pubmed-author:PeterhansEE | lld:pubmed |
| pubmed-article:8218933 | pubmed:author | pubmed-author:RoyD JDJ | lld:pubmed |
| pubmed-article:8218933 | pubmed:author | pubmed-author:FiskerstrandC... | lld:pubmed |
| pubmed-article:8218933 | pubmed:author | pubmed-author:SarganD RDR | lld:pubmed |
| pubmed-article:8218933 | pubmed:author | pubmed-author:GreenI RIR | lld:pubmed |
| pubmed-article:8218933 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8218933 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:8218933 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8218933 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8218933 | pubmed:pagination | 213-23 | lld:pubmed |
| pubmed-article:8218933 | pubmed:dateRevised | 2009-9-29 | lld:pubmed |
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| pubmed-article:8218933 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8218933 | pubmed:articleTitle | Expression and characterization of bioactive recombinant ovine TNF-alpha: some species specificity in cytotoxic response to TNF. | lld:pubmed |
| pubmed-article:8218933 | pubmed:affiliation | Department of Veterinary Pathology, University of Edinburgh, UK. | lld:pubmed |
| pubmed-article:8218933 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8218933 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8218933 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
