Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1993-12-15
|
| pubmed:abstractText |
We have expressed and partially purified recombinant ovine tumour necrosis factor alpha (rovTNF-alpha) using a yeast Ty, virus like particle, expression system. RovTNF-alpha is at least as active as recombinant human TNF-alpha (rhTNF-alpha) in two different bio-assays performed on ovine material, whilst approximately 1000-fold more rovrTNF-alpha than rhTNF-alpha is required to induce the same level of cytotoxicity in TNF-sensitive murine cell lines L929 and WEHI 164 clone 13. When cytotoxic assays are performed on the porcine TNF sensitive cell line PK(15)-1512, rovTNF-alpha shows about 2 logs greater activity than on murine cells, whilst rhTNF-alpha is about 1 log more active. A monoclonal antibody, raised against rovTNF-alpha, has been used to demonstrate the presence of nanogram amounts of an appropriately sized glycoprotein to be native ovine TNF-alpha in supernants of LPS stimulated ovine alveolar macrophages. These samples show no detectable cytotoxicity to L929 cells, although they show activity attributable to TNF-alpha (through neutralization by a polyclonal antiserum raised to rovTNF-alpha) in an assay on ovine material. The relative lack of activity on murine cells helps to explain previous reports of inability to assay native ovine TNF-alpha using these cells, in spite of their routine use to assay TNF-alpha from several other species. The sequence features in ovine TNF-alpha which might reduce its affinity for the murine TNF type 1 receptor are discussed.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
1043-4666
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
213-23
|
| pubmed:dateRevised |
2009-9-29
|
| pubmed:meshHeading |
pubmed-meshheading:8218933-Amino Acid Sequence,
pubmed-meshheading:8218933-Animals,
pubmed-meshheading:8218933-Base Sequence,
pubmed-meshheading:8218933-Blotting, Western,
pubmed-meshheading:8218933-Cartilage, Articular,
pubmed-meshheading:8218933-Cell Line,
pubmed-meshheading:8218933-Cell Survival,
pubmed-meshheading:8218933-DNA Primers,
pubmed-meshheading:8218933-Fibrosarcoma,
pubmed-meshheading:8218933-Humans,
pubmed-meshheading:8218933-Lymphocyte Activation,
pubmed-meshheading:8218933-Macrophage Activation,
pubmed-meshheading:8218933-Macrophages,
pubmed-meshheading:8218933-Mice,
pubmed-meshheading:8218933-Molecular Sequence Data,
pubmed-meshheading:8218933-Molecular Weight,
pubmed-meshheading:8218933-Recombinant Fusion Proteins,
pubmed-meshheading:8218933-Recombinant Proteins,
pubmed-meshheading:8218933-Sequence Homology, Amino Acid,
pubmed-meshheading:8218933-Sheep,
pubmed-meshheading:8218933-Species Specificity,
pubmed-meshheading:8218933-Swine,
pubmed-meshheading:8218933-T-Lymphocytes,
pubmed-meshheading:8218933-Tumor Cells, Cultured,
pubmed-meshheading:8218933-Tumor Necrosis Factor-alpha
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Expression and characterization of bioactive recombinant ovine TNF-alpha: some species specificity in cytotoxic response to TNF.
|
| pubmed:affiliation |
Department of Veterinary Pathology, University of Edinburgh, UK.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|